Transposon-specified site-specific recombination.
Transposon-specified site-specific recombination.
复制标题
转座子指定的位点特异性重组。
DOI：
10.1073/pnas.79.1.46
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发表时间：
1982
影响因子：
11.1
通讯作者：
Sherratt,D
中科院分区：
文献类型：
--
作者：
Kitts,P;Symington,L;Burke,M;Reed,R;Sherratt,D
包含转座因子的两个拷贝的协整DNA分子似乎是转座过程中的中间体。这些结构通过位点特异性重组来解决，从而产生正常的转位最终产物。转座因子γ δ (Tn1000)合成了一个与Tn1/3tnpR蛋白可互换的产物，促进Tn1/3位点特异性重组。这些数据支持这样的假设，即包含Tn1/3直接重复拷贝的协整是Tn1/3复制子间转位的强制性中间产物。此外，我们在这里表明，该反应是独立于元件编码的tnpA基因产物。指定抗汞性的Tn501也作为复制子间转位的中间产物产生协整体。tn501指定的重组活性的出现可以作用于这些协整体，需要细胞在Hg2+存在下生长。
Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.