Phenotypic characterization of human decidual macrophages
Phenotypic characterization of human decidual macrophages
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DOI：
10.1046/j.1365-2249.2003.02092.x
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发表时间：
2003-03-01
影响因子：
4.6
通讯作者：
Lassila, O
中科院分区：
文献类型：
--
作者：
Heikkinen, J;Möttönen, M;Lassila, O
怀孕是对免疫系统的挑战，免疫系统不仅要保护母亲和胎儿免受入侵病原体的侵袭，还要保持对胎儿的免疫耐受性。然而，母体蜕膜组织中抑制局部免疫反应的机制却知之甚少。我们研究了蜕膜CD14(+)巨噬细胞，它在维持对发育中胎儿的耐受性方面可能很重要。蜕膜巨噬细胞表达人类白细胞抗原-DR，但共刺激分子CD86水平低于妊娠和非妊娠妇女外周血CD14(+)单核细胞。蜕膜巨噬细胞自发产生高水平的白介素10。我们的发现提示蜕膜巨噬细胞可能代表抑制性APC。支持这一结论的吲哚胺2，3-双加氧酶(IDO)在蜕膜巨噬细胞中表达，被认为在妊娠过程中具有免疫抑制作用。此外，蜕膜巨噬细胞在IL-4+GM-CSF的作用下不能分化为树突状细胞。这些结果提示蜕膜巨噬细胞在母胎界面具有免疫抑制功能。
Pregnancy is a challenge to the immune system, which not only has to protect the mother and the fetus from invading pathogens but to also maintain immunological tolerance against the fetus. However, the mechanisms inhibiting local immune responses in the maternal decidual tissue are poorly understood. We have studied decidual CD14(+) macrophages, which may be important in the maintenance of a tolerance against the developing fetus. Decidual macrophages expressed HLA-DR, but lower levels of costimulatory molecule CD86 than peripheral blood CD14(+) monocytes from pregnant and non-pregnant women. Decidual macrophages produced spontaneously high levels of interleukin-10. Our findings suggest that decidual macrophages could represent an inhibitory type of APCs. Supporting this conclusion indoleamine 2,3-dioxygenase (IDO), suggested to have an immunosuppressive role in pregnancy, was expressed in decidual macrophages. Furthermore, decidual macrophages were not able to differentiate into dendritic cells under the influence of IL-4 + GM-CSF. These results suggest an immunoinhibitory function of decidual macrophages at the maternal-fetal interface.