Detection of enteroviral RNA in idiopathic dilated cardiomyopathy and other human cardiac tissues.
Detection of enteroviral RNA in idiopathic dilated cardiomyopathy and other human cardiac tissues.
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特发性扩张型心肌病和其他人体心脏组织中肠道病毒 RNA 的检测。
DOI：
10.1172/jci115830
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Billingham,ME
中科院分区：
文献类型：
--
作者：
Weiss,LM;Liu,XF;Chang,KL;Billingham,ME
肠道病毒被认为是特发性扩张型心肌病的可能原因之一。我们使用聚合酶链式反应方法鉴定肠道病毒RNA，试图为肠道病毒在特发性扩张型心肌病发病机制中的作用提供证据。该方法被证明可以识别多种肠道病毒，灵敏度高达0.1-1个空斑形成单位/克组织。11例特发性扩张型心肌病患者中5例(45%)肠道病毒核酸序列阳性，24例其他心脏疾病(包括正常心脏)中9例(38%)肠道病毒核酸序列阳性，8例乳腺癌对照病例均为阴性。这些结果表明，正常和异常的心脏都可能是潜伏的或低级别的持续性肠道病毒感染的部位，仅仅是肠道病毒核酸序列的存在与特发性扩张型心肌病没有特殊的联系。
Enteroviruses have been considered to be a possible cause of idiopathic dilated cardiomyopathy. We used a polymerase chain reaction methodology for the identification of enteroviral RNA in an attempt to provide evidence of a role for enteroviruses in the pathogenesis of idiopathic dilated cardiomyopathy. The methodology was shown to identify a wide variety of enteroviruses with a sensitivity up to 0.1-1 plaque-forming units/gram of tissue. 5 of 11 cases (45%) of idiopathic dilated cardiomyopathy, as well as 9 of 24 cases (38%) of a wide variety of other cardiac conditions (including normal heart), were positive for enteroviral nucleic acid sequences; all eight control cases of breast carcinoma tested were negative. These results suggest that both the normal and abnormal heart may represent a site of latent or low-grade persistent enteroviral infection, and that the mere presence of enteroviral nucleic acid sequences is not specifically associated with idiopathic dilated cardiomyopathy.Images