Ubiquitin-EP52 fusion protein homologs from Trypanosoma brucei.
Ubiquitin-EP52 fusion protein homologs from Trypanosoma brucei.
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来自布氏锥虫的泛素-EP52 融合蛋白同源物。
DOI：
10.1093/nar/18.23.7181
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发表时间：
1990
影响因子：
14.9
通讯作者：
Campbell,DA
中科院分区：
文献类型：
--
作者：
Wong,S;Morales,TH;Campbell,DA
利用布鲁氏锥虫泛素探针筛选布鲁氏锥虫原环cDNA文库，鉴定出两个编码泛素- ep52同源物的克隆。两个克隆的开放阅读名（图1）预测一个相同的128个氨基酸序列。其氨基末端的76个氨基酸与源自布氏体多泛素基因的泛素单体相同(1)。羧基末端的52个氨基酸在其他细胞类型中有同源物（图2），它们可能作为与核糖体小亚基相关的离散多肽存在(3)。在酵母中，同源物与核糖体的生物发生有关(3)。这两个cDNA克隆在编码区包含两个碱基变化，可以通过3'非编码区序列差异进一步区分；因此它们代表了不同基因的转录本。
Screening of a Trypanosoma brucei procyclic cDNA library with a T. brucei ubiquitin probe identified two clones which encoded ubiquitin-EP52 homologs. The open reading fames of the two clones (Figure 1) predict an identical, 128 amino acid sequence. The amino terminal 76 amino acids are identical to the ubiquitin monomer derived from a T. brucei polyubiquitin gene (1). The carboxy terminal 52 amino acids have homologs in other cell types (Figure 2) wherethey may exist as a discrete polypeptide associated with the small subunit of the ribosome (3). In yeast, the homologs have been implicated in ribosome biogenesis (3). The two cDNA clones contain two base changes in the coding region and can be further distinguished by sequence divergence in the 3'non-coding region; they therefore represent transcripts from different genes.