A Monte Carlo-based model of gold nanoparticle radiosensitization accounting for increased radiobiological effectiveness
A Monte Carlo-based model of gold nanoparticle radiosensitization accounting for increased radiobiological effectiveness
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DOI：
10.1088/0031-9155/58/10/3075
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发表时间：
2013-05-21
影响因子：
3.5
通讯作者：
Pignol, J-P
中科院分区：
文献类型：
--
作者：
Lechtman, E.;Mashouf, S.;Pignol, J-P
使用金纳米颗粒（AuNPs）的放射致敏作用已被证明与细胞系、辐照能量、AuNP大小、浓度和细胞内定位有很大差异。我们开发了一个基于蒙特卡罗的AuNP放射敏化预测模型（ARP），该模型考虑了纳米尺度上的详细能量沉积。该模型与实验细胞存活和宏观剂量增强预测进行了比较。用300 kVp光子源照射PC-3前列腺癌细胞后，观察细胞培养基中是否存在AuNPs。进行了详细的蒙特卡罗模拟，产生了逃离aunp的光电产品的单个轨迹，并在模型细胞核的纳米尺度体素中对能量沉积进行了评分。在我们的预测模型中，细胞存活率是通过将辐射致死事件密度除以细胞核体积来计算的。实验观察到AuNP放射增敏，增敏剂增强比（SER）为1.21 +/- 0.13。使用ARP模型和宏观增强模型估计的SERs分别为1.20 +/- 0.12和1.07 +/- 0.10。在假设AuNP定位于细胞核内的情况下，ARP模型预测SER为1.29 +/- 0.13，表明AuNP细胞内定位对放射致敏的影响。
Radiosensitization using gold nanoparticles (AuNPs) has been shown to vary widely with cell line, irradiation energy, AuNP size, concentration and intracellular localization. We developed a Monte Carlo-based AuNP radiosensitization predictive model (ARP), which takes into account the detailed energy deposition at the nano-scale. This model was compared to experimental cell survival and macroscopic dose enhancement predictions. PC-3 prostate cancer cell survival was characterized after irradiation using a 300 kVp photon source with and without AuNPs present in the cell culture media. Detailed Monte Carlo simulations were conducted, producing individual tracks of photoelectric products escaping AuNPs and energy deposition was scored in nano-scale voxels in a model cell nucleus. Cell survival in our predictive model was calculated by integrating the radiation induced lethal event density over the nucleus volume. Experimental AuNP radiosensitization was observed with a sensitizer enhancement ratio (SER) of 1.21 +/- 0.13. SERs estimated using the ARP model and the macroscopic enhancement model were 1.20 +/- 0.12 and 1.07 +/- 0.10 respectively. In the hypothetical case of AuNPs localized within the nucleus, the ARP model predicted a SER of 1.29 +/- 0.13, demonstrating the influence of AuNP intracellular localization on radiosensitization.