Study on the anti-endotoxin effect of sinomenine using an Agilent genome array
Study on the anti-endotoxin effect of sinomenine using an Agilent genome array
复制标题
利用安捷伦基因组芯片研究青藤碱的抗内毒素作用
DOI：
10.1093/qjmed/hcx234
复制
发表时间：
2018-03
影响因子：
13.3
通讯作者：
He K.
中科院分区：
文献类型：
--
作者：
Hu Y.;Li B.;Wen L.;He K.
背景
内毒素是导致发热、腹泻、炎症、水肿、凝血、休克和与革兰氏阴性细菌感染相关的其他综合征发生的重要因素。迄今为止，没有有效的治疗内毒素血症。
目的
本研究的目的是通过微阵列分析来表征青藤碱处理和脂多糖（LPS）诱导的内皮细胞中差异表达的基因，并确定青藤碱的潜在药理活性。
设计
收集5个处理组（n = 3）的培养细胞。研究对象：提取猪总RNA，应用Agilent Porcine 4 × 44 K全基因组芯片进行分析.
方法
应用京都基因和基因组百科全书和基因本体软件筛选和分析差异调控基因。
结果
结果显示，治疗组与LPS组相比，共检测到723条差异表达基因，其中上调基因410条，下调基因313条。10个基因可能是LPS发病机制中的关键调控基因，其中5个上调基因（ARG1、TLR2、IL1A、VCAM 1、DKK 3）和5个下调基因（HABP2、ID1、CHDH、GPX3、PTGFR）主要参与炎症反应、血管病变、代谢过程和细胞周期等生物学过程。IL1A和FMO3被认为是有效的靶基因。
结论
全局基因表达谱分析表明，青藤碱可能有效地调节炎症反应，作为未来抗内毒素治疗的一部分。
Background
Endotoxin is a significant contributing factor underlying the occurrence of fever, diarrhea, inflammation, edema, coagulation, shock and other syndromes associated with gram-negative bacterial infections. To date, there is no effective treatment for endotoxemia.
Aim
The aim of this study was to characterize differentially expressed genes in sinomenine-treated and lipopolysaccharide (LPS)-induced endothelial cells by microarray analysis and to determine the potential pharmacological activity of sinomenine.
Design
The cultured cells of five treatment groups (n = 3) were collected. Participants: total RNA was extracted and subjected to Agilent Porcine 4 × 44 K whole genome microarray.
Methods
Kyoto encyclopedia of genes and genomes and gene ontology software were applied to screen and analyze differentially regulated genes.
Results
The results showed that 723 differentially regulated genes were identified including 410 up-regulated genes and 313 down-regulated genes in therapy group vs. LPS group. Ten genes may be key controlled genes in the pathogenesis of LPS, including five up-regulated genes (ARG1, TLR2, IL1A, VCAM1, DKK3) and five down-regulated genes (HABP2, ID1, CHDH, GPX3, PTGFR), which primarily contribute to biological processes such as inflammatory response, vascular lesion, metabolic process and cell cycle. IL1A and FMO3 were considered as potent target genes.
Conclusion
Global gene expression profile analysis showed that sinomenine might effectively be useful to regulate inflammatory responses as part of future anti-endotoxin therapies.
登录
查看更多内容
青藤碱通过腺苷 A(2A) 受体信号传导防止脂多糖诱导的小鼠急性肺损伤。
影响因子：
3.7
作者：
Li J;Zhao L;He X;Zeng YJ;Dai SS
通讯作者：
Dai SS
影响因子：
3.6
作者：
E. Thorgersen;A. Macagno;C. Rossetti;T. Mollnes
通讯作者：
E. Thorgersen;A. Macagno;C. Rossetti;T. Mollnes
DOI：
10.1152/ajpendo.00220.2002
发表时间：
2002-11
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
R. Orellana;P. M. O'Connor;Hanh V. Nguyen;Jill A. Bush;A. Suryawan;M. Thivierge;M. Fiorotto;T. Davis
通讯作者：
R. Orellana;P. M. O'Connor;Hanh V. Nguyen;Jill A. Bush;A. Suryawan;M. Thivierge;M. Fiorotto;T. Davis
DOI：
10.1124/jpet.102.035758
发表时间：
2002-11
影响因子：
3.5
作者：
Taiyo Suganuma;K. Irie;E. Fujii;T. Yoshioka;T. Muraki
通讯作者：
Taiyo Suganuma;K. Irie;E. Fujii;T. Yoshioka;T. Muraki
影响因子：
6.7
作者：
Gao, J.;Zhao, W. X.;Chen, Z. Q.
通讯作者：
Chen, Z. Q.