CYTOPLASMIC SYNTHESIS OF PLASTID POLYPEPTIDES MAY BE CONTROLLED BY PLASTID-SYNTHESIZED RNA
CYTOPLASMIC SYNTHESIS OF PLASTID POLYPEPTIDES MAY BE CONTROLLED BY PLASTID-SYNTHESIZED RNA
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DOI：
10.1038/279816a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
HAGEMANN, R
中科院分区：
文献类型：
--
作者：
BRADBEER, JW;ATKINSON, YE;HAGEMANN, R
一般认为，一些质体多肽由质体DNA编码，其余部分由核DNA编码，一些质体多肽在质体核糖体上合成，其余部分在细胞质核糖体上合成1,2。大麦（Hordeum vulgareL）两个突变系的研究。“白体”（M4205）和“萨斯卡通”)有助于理解质体和核细胞质系统的整合。在这两个品系中，核突变表现为质体DNA的不可逆突变，产生缺乏色素的质体，其分离导致叶片呈白色条纹，随后形成纯白色叶片和芽3,4。白色叶片不含质体核糖体，在质体中检测不到蛋白质合成，但细胞质核糖体的含量正常5,6。白叶提取物的免疫电泳没有检测到质体合成的核酮糖-二磷酸羧化酶/加氧酶的大亚基或胞质合成的小亚基，也没有检测到偶联因子CF1，它有三个质体合成和两个胞质合成的亚基，也没有检测到胞质合成的铁氧化还原蛋白- nadp +还原酶6,7。我们在此报告了两种细胞质合成的质体酶8，磷脂激酶（EC 2.7.1.19）和d -甘油醛-3-磷酸：NADP+氧化还原酶（磷酸化）（EC 1.2.1.13），在白色叶片中出现的数量大大减少，并推断了质体如何控制质体多肽的细胞质合成。
IT is generally agreed that some plastid polypeptides are coded by plastid DNA and the remainder by nuclear DNA, and that some plastid polypeptides are synthesised on plastid ribosomes and the remainder on cytoplasmic ribosomes1,2. Studies on two mutant lines of barley (Hordeum vulgareL. ‘albostrians’ (M4205) and ‘Saskatoon’) have helped the understanding of the integration of the plastid and nucleocytoplasmic systems. In both lines a nuclear mutation is expressed as an irreversible mutation of the plastid DNA yielding pigment-deficient plastids whose segregation leads to white-striped leaves and later to pure white leaves and shoots3,4. The white leaves do not contain plastid ribosomes and no protein synthesis can be detected in the plastids, although cytoplasmic ribosomes are present in normal amounts5,6. Immunoelectrophoresis of extracts of white leaves has failed to detect either the plastid-synthesised large subunit or the cytoplasmically synthesised small subunit of ribulose-bisphosphate carboxylase/oxygenase, or coupling factor CF1, which has three plastid-synthesised and two cytoplasmically synthesised subunits, or the cytoplasmically synthesised ferredoxin-NADP+reductase6,7. We report here that two cytoplasmically synthesised plastid enzymes8, phosphoribulokinase (EC 2.7.1.19) andD-glyceraldehyde-3-phosphate: NADP+oxidoreductase (phosphorylating) (EC 1.2.1.13), occur in considerably reduced amounts in white leaves and deduce how the plastid may control the cytoplasmic synthesis of plastid polypeptides.