BIOCHEMICAL-EVIDENCE OF RECOMBINATION WITHIN THE UNSEGMENTED RNA GENOME OF APHTHOVIRUS
BIOCHEMICAL-EVIDENCE OF RECOMBINATION WITHIN THE UNSEGMENTED RNA GENOME OF APHTHOVIRUS
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DOI：
10.1128/jvi.41.1.66-77.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
NEWMAN, JWI
中科院分区：
文献类型：
--
作者：
KING, AMQ;MCCAHON, D;NEWMAN, JWI
利用感染中心重组试验，对来自同一株口疮病毒的四对不同的温度敏感突变体进行了杂交。每一个亲本突变体携带一个影响病毒编码的多肽的等电点的标记，杂交后代，能够在非允许的温度下生长，这些标记进行筛选。多肽电荷被证明是一个稳定的，可遗传的字符，和4个杂交产生的遗传重组体，具有来自双亲的多肽标记。多肽的肽指纹图谱和高分辨率等电聚焦排除了这些病毒是由突变产生的解释。
Four different pairs of temperature-sensitive mutants, derived from the same strain of aphthovirus, were crossed by using an infectious center recombination test. Each parental mutant carried an unselected marker affecting the isoelectric point of a virus-coded polypeptide; progeny of the crosses, able to grow at the nonpermissive temperature, were screened for these unselected markers. Polypeptide charge was shown to be a stable, inheritable character, and 3 of 4 crosses yielded genetic recombinants that possessed the polypeptide marker from both parents. Peptide fingerprinting and high-resolution isoelectric focusing of the polypeptides ruled out the explanation that these viruses were generated by mutation.