The multidrug efflux pump NorA is not required for salicylate-induced reduction in drug accumulation by Staphylococcus aureus
The multidrug efflux pump NorA is not required for salicylate-induced reduction in drug accumulation by Staphylococcus aureus
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DOI：
10.1016/s0924-8579(02)00162-0
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发表时间：
2002-09-01
影响因子：
10.8
通讯作者：
Gustafson, JE
中科院分区：
文献类型：
--
作者：
Price, CTD;Kaatz, GW;Gustafson, JE
水杨酸盐存在下金黄色葡萄球菌的生长会导致环丙沙星和乙锭减少。积累并增加对乙锭的抵抗力。水杨酸盐诱导的环丙沙星积累的减少是与能量无关的，而水杨酸盐诱导的乙锭积累和流出的改变是质子动力依赖性的。 NorA 是一种内在的多药外排泵，有助于提高氟喹诺酮和乙锭的内在水平，以及金黄色葡萄球菌的耐药性。 NorA 抑制剂利血平不会显着影响水杨酸盐诱导环丙沙星和乙锭耐药性增加的能力。 NorA 失活不会改变水杨酸盐诱导环丙沙星和乙锭耐药水平增加以及环丙沙星积累减少的能力。这些数据表明，NorA 并不是金黄色葡萄球菌水杨酸盐诱导的多药耐药机制所绝对需要的。 (C) 2002 年 Elsevier Science B.V. 和国际化疗协会。版权所有。
Growth of Staphylococcus aureus in the presence of salicylate leads to reduced ciprofloxacin and ethidium. accumulation and increased resistance to ethidium. Salicylate induced reduction in ciprofloxacin accumulation is energy-independent while salicylate induced alterations in ethidium accumulation and efflux is proton motive force-dependent. NorA is an intrinsic multidrug efflux pump that contributes to intrinsic levels of fluoroquinolone and ethidium, resistance in S. aureus. The NorA inhibitor reserpine did not dramatically affect the ability of salicylate to induce increased ciprofloxacin and ethidium resistance. Inactivation of norA did not alter the ability of salicylate to induce increased ciprofloxacin and ethidium resistance levels and a reduction in ciprofloxacin accumulation. These data demonstrate that NorA is not absolutely required for the salicylate-inducible multidrug resistance mechanism of S. aureus. (C) 2002 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.