Neither the presence of ica locus, nor in vitro-biofilm formation ability is a crucial parameter for some Staphylococcus epidermidis strains to maintain an infection in a guinea pig tissue cage model
Neither the presence of ica locus, nor in vitro-biofilm formation ability is a crucial parameter for some Staphylococcus epidermidis strains to maintain an infection in a guinea pig tissue cage model
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DOI：
10.1016/j.micpath.2006.09.001
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Jabbouri, Said
中科院分区：
文献类型：
--
作者：
Chokr, Ali;Leterme, Damien;Jabbouri, Said
表皮葡萄球菌的发病机制被认为是基于它通过形成生物膜来定植医疗器械的能力。生物膜的形成部分是由由icaADBC操纵子编码的多糖胞间黏附素(PIA)介导的。我们已经在体外研究了一些凝固酶阴性葡萄球菌(CONS)临床分离株的生物膜形成(B+/-)、ICA基因座(1+/-)和PTA产生(P+/-)之间的相关性。在这里，我们使用皮下植入组织笼的豚鼠模型来评估13个I和P参数在9个表皮葡萄球菌和1个肉芽葡萄球菌在体内发展和维持感染能力方面的意义。利用临床分离株和一株模式菌株，我们证明了“B+，I+，P+”型赋予体内维持感染的能力。令人惊讶的是，相反的类型“B-，I-，P-”在临床和共生分离株的测试中，感染率从25%到60%不等。在本模型中，其他具有“B+I+，P-”型的临床分离株不能引起感染。这些结果表明，根据菌株的不同，定植组织笼的能力可能与形成生物膜的能力无关。(C)2006爱思唯尔有限公司。保留所有权利。
The pathogenesis of Staphylococcus epidermidis is thought to be based on its capacity to colonize medical devices by forming a biofilm. Biofilm formation is in part mediated by the polysaccharide intercellular adhesin (PIA), which is encoded by the icaADBC operon. We have previously investigated in vitro the correlation existing between biofilm formation (B +/-), presence of ica locus (1 +/-) and PTA production (P +/-) in some clinical isolates of coagulase-negative staphylococci (CoNS). Here, we used a guinea pig model of subcutaneous implanted tissue cages to assess the implication of 13 I and P parameters in the capacity of nine S. epidermidis and one S. carnosus strains to develop and maintain an infection in vivo. Using clinical isolates and a model strain of S. epidermidis, we showed that the "B+, I+, P+" type confers the ability to maintain an infection in vivo. Surprisingly, the opposite type "B-, I-, P-" tested with clinical and commensal isolates, presented infection rates ranging from 25% to 60%. Other clinical isolates having a "B+ I+, P-" type, were not able to cause an infection in the present model. These results showed that, depending on the strains the capacity to colonize the tissue cage might be independent of the ability to form biofilm. (c) 2006 Elsevier Ltd. All rights reserved.