Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder
Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder
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DOI：
10.1016/j.eururo.2009.12.031
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发表时间：
2010-08-01
期刊：
影响因子：
23.4
通讯作者：
Nishizawa, Osamu
中科院分区：
文献类型：
--
作者：
Minagawa, Tomonori;Imamura, Tetsuya;Nishizawa, Osamu
背景资料：人羊膜间充质细胞（human amniotic mesenchymal cells，HAMCs）的多能性已被报道，但HAMCs在尿路再生中的作用尚不清楚。目的：本研究的目的是确定来源于HAMCs的细胞是否支持冷冻损伤小鼠膀胱的结构和功能重建。从羊膜中收获HAMCs，并将细胞培养7天，然后注射到冷冻损伤的裸鼠膀胱壁中。干预：植入前3天，冷冻损伤后膀胱壁30 s。将培养的HAMC衍生细胞（0.5 × 10（5）个细胞/50 μ l）植入损伤区域。对照膀胱接受无细胞注射。于细胞植入后1、2、4、6周，取膀胱组织行α-平滑肌肌动蛋白（SMA）免疫组织化学染色。用抗人核抗体（HuNu）检测HAMC来源的细胞。分别，膀胱肌条进行了检查收缩反应钾。结果和限制：在植入后1周，HAMC衍生的细胞，这是由胡奴检测，分化成肌层组成的SMA阳性细胞。植入后2 ~ 6周，出现了丰富的SMA阳性和HuNu阳性细胞层。在对照组膀胱中，在1周时，很少SMA阳性细胞保留在损伤区域，但到6周时，更多的SMA阳性细胞存在。1周时，细胞植入膀胱对钾的收缩反应显著高于对照注射膀胱。对照注射膀胱也恢复了6周，但恢复的速度较慢。结论：冷冻损伤的小鼠膀胱植入HAMC衍生细胞恢复形态和功能的速度比对照注射膀胱。(C)2009年欧洲泌尿外科协会。由Elsevier B出版。V.保留所有权利。
Background: The multipotency of human amniotic mesenchymal cells (HAMCs) has been reported, but the role of HAMCs in urinary tract regeneration is unknown.Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders.Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice.Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated.Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium.Results and limitations: At 1 wk after implantation, the HAMC-derived cells, which were detected by HuNu, differentiated into muscular layers composed of SMA-positive cells. From 2 to 6 wk after implantation, abundant layers of SMA-positive and HuNu-positive cells developed. In control bladders, few SMA-positive cells remained at the injured regions at 1 wk, but by 6 wk, more were present. At 1 wk, the contractile responses to potassium of the cell-implanted bladders were significantly higher than those of the control-injected ones. Control-injected bladders also recovered by 6 wk, but the rate of recovery was slower.Conclusions: Freeze-injured mouse bladders implanted with HAMC-derived cells recovered morphology and function faster than control-injected bladders. (C) 2009 European Association of Urology. Published by Elsevier B. V. All rights reserved.