Study on Diagnostic Values of Astrocyte Elevated Gene 1 (AEG-1) and Glypican 3 (GPC-3) in Hepatocellular Carcinoma
Study on Diagnostic Values of Astrocyte Elevated Gene 1 (AEG-1) and Glypican 3 (GPC-3) in Hepatocellular Carcinoma
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DOI：
10.1093/ajcp/aqz086
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发表时间：
2019-11-01
影响因子：
3.5
通讯作者：
Yu, Jiangzhou
中科院分区：
文献类型：
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作者：
Cao, Wenqing;Sharma, Meenal;Yu, Jiangzhou
目的：目的：探讨AEG-1和GPC-3在肝细胞癌（HCC）诊断中的应用价值。方法：应用免疫组化方法对HCC、癌旁组织（ANT）和不典型增生结节（DN）进行AEG-1和GPC-3的检测。在HCC中，AEG-1和GPC-3的阳性率分别为92%和54%。ANT中AEG-1和GPC-3阳性率分别为16.2%和7.6%。AEG-1染色主要是弥漫性的，而GPC-3经常显示局灶性染色。AEG-1单药敏感性高，但特异性和准确性低。另一方面，GPC-3显示出高特异性，但低灵敏度和准确性。当仅弥漫染色被认为是阳性时，两种染色的组合使敏感性、特异性和准确性分别提高到94.6%、89.5%和90.5%。AEG-1和GPC-3联合检测可提高肝癌的早期诊断率。
Objectives: To investigate the diagnostic potential of AEG-1 and GPC-3 in hepatocellular carcinoma (HCC).Methods: AEG-1 and GPC-3 immunohistochemistry were performed on HCC, adjacent nontumor tissue (ANT), and dysplastic nodules (DN).Results: H score of AEG-1 or GPC-3 in HCC was significantly higher than in ANT or DN. In HCC, 92% and 54% showed AEG-1 and GPC-3 positivity, respectively. In ANT, 16.2% were AEG-1 and 7.6% GPC-3 positive. AEG-1 staining was mostly diffuse, whereas GPC-3 frequently showed focal staining. AEG-1 alone showed high sensitivity but low specificity and accuracy. GPC-3, on the other hand, showed high specificity but low sensitivity and accuracy. Combination of both stains boosted the sensitivity, specificity, and accuracy to 94.6%, 89.5%, and 90.5%, respectively, when only diffuse staining was considered as positive.Conclusions: AEG-1 or GPC-3 alone seemed not an ideal marker for HCC. The combination of AEG-1 and GPC-3 might improve early diagnosis of HCC.