Chemical profiling and antiproliferative effect of essential oils of two Araucaria species cultivated in Egypt
Chemical profiling and antiproliferative effect of essential oils of two Araucaria species cultivated in Egypt
复制标题
DOI：
10.1016/j.indcrop.2018.03.051
复制
发表时间：
2018-08-01
影响因子：
5.9
通讯作者：
Ayoub, Iriny M.
中科院分区：
文献类型：
--
作者：
Elkady, Wafaa M.;Ayoub, Iriny M.
南洋杉是万年青针叶树的一个小属，广泛用于装饰和木材用途。Araucaroia物种富含精油。本文对南洋杉（Araucaria heterophylla（Salisb.）和南洋杉的挥发油进行了分析，使用GC和GC/MS。细胞毒性的三种人癌细胞株，使用MTT法进行评估。29个化合物在A. Heterophylla精油中鉴定出43种，而A.毛蕊花和毛蕊花分别占挥发油总成分的95.16%和81.06%。α-蒎烯（70.85%）、D-柠檬烯（4.26%）和大根香叶烯D（2.99%）是A.异叶植物A.中以Beyerene（35.65%）、transnerolidol（13.66%）和γ-榄香烯（6.09%）为主;比德威利。两种油对所有测试的癌细胞系（Hep-G2、MCF-7和Caco-2）均显示出有效的细胞毒性，表现出A的IC 50值为0.70、3.20和1.10 μ g/ml。Heterophylla为1.67、1.10和1.32 μ g/ml;比德威利分别。Caspase-3激活表明A.鬼针草精油通过半胱天冬酶依赖性细胞凋亡介导。这些结果表明，A。heterophylla和A. Bidwilli提供了与其单个组分的协同作用有关的有希望的抗癌候选药物。
Araucaria is a small genus of evergreen coniferous trees, widely used for ornamental and timber purposes. Araucaroia species are rich in essential oils. In this study, the essential oils obtained from the foliage of Araucaria heterophylla (Salisb.) and Araucaria bidwillii Hook were analyzed using GC and GC/MS. Cytotoxicity of both essential oils was assessed on three human cancer cell lines using MTT assay. Twenty nine compounds were annotated in A. heterophylla essential oil, whereas forty three were identified in A. bidwillii accounting for 95.16% and 81.06% of the total volatile oil composition, respectively. alpha-Pinene (70.85%), D-limonene (4.26%) and germacrene D (2.99%) represented the major compounds in A. heterophylla. Beyerene (35.65%), transnerolidol (13.66%) and gamma-elemene (6.09%) dominated in A. bidwillii. Both oils showed potent cytotoxicity against all tested cancer cell lines (Hep-G2, MCF-7 and Caco-2) exhibiting IC50 values of 0.70, 3.20 and 1.10 mu g/ml for A. heterophylla; and 1.67, 1.10 and 1.32 mu g/ml for A. bidwillii, respectively. Caspase-3 activation indicated that the cytotoxicity of A. bidwillii essential oil is mediated via caspase-dependent apoptosis. These findings suggest that the essential oils of A. heterophylla and A. bidwilli offer promising anticancer drug candidates pertaining to the synergistic effects of their individual components.