The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue
The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue
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DOI：
10.1038/ijo.2011.223
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发表时间：
2012-10-01
影响因子：
4.9
通讯作者：
Stich, V.
中科院分区：
文献类型：
--
作者：
Kovacova, Z.;Tencerova, M.;Stich, V.
目的：肥胖者的低脂联素血症与胰岛素抵抗、糖尿病和动脉粥样硬化有关。本研究的目的是调查分泌脂联素及其多聚体异构体的外植体来自皮下脂肪组织（SAT）和内脏脂肪组织（VAT）在肥胖和non-obese subjects.DESIGN：配对样本的SAT和VAT和血液样本从23例（10非肥胖和13肥胖）接受择期腹部手术。采用酶联免疫吸附试验和非变性蛋白质印迹法分别测定了SAT和VAT外植体条件培养液中脂联素总量和脂联素亚型。结果：肥胖者血浆脂联素总量低于非肥胖者（P
OBJECTIVE: Hypoadiponectinemia observed in obesity is associated with insulin resistance, diabetes and atherosclerosis. The aim of the present study was to investigate secretion of adiponectin and its multimeric isoforms by explants derived from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in obese and non-obese subjects.DESIGN: Paired samples of SAT and VAT and blood samples were obtained from 23 subjects (10 non-obese and 13 obese) undergoing elective abdominal surgery. Total adiponectin quantities and adiponectin isoforms were measured in conditioned media of explants derived from SAT and VAT using enzyme-linked immunosorbent assay and non-denaturing western blot, respectively.RESULTS: Total adiponectin plasma levels were lower in obese than in non-obese subjects (P