ANTIBODY-SECRETING CELLS IN THE EVALUATION OF THE IMMUNOGENICITY OF AN ORAL VACCINE
ANTIBODY-SECRETING CELLS IN THE EVALUATION OF THE IMMUNOGENICITY OF AN ORAL VACCINE
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DOI：
10.1016/0264-410x(90)90088-4
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发表时间：
1990-08-01
期刊：
影响因子：
5.5
通讯作者：
KANTELE, A
中科院分区：
文献类型：
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作者：
KANTELE, A
通过计数外周血中的特异性抗体分泌细胞（ASC）研究了对不同剂量方案的口服伤寒沙门氏菌Ty 21 a活疫苗的免疫应答，认为这些细胞已被粘膜表面上的疫苗抗原刺激，并正在返回这些位点进行局部抗体分泌。四组受试者用三种（3 × 10）疫苗接种。S）、两个（2 × S）、两个（2 × S）、两个（2 × S）、两个（2 × S）。S）或一个（1 × S）。S）剂量的悬浮液配制的疫苗，或用三个剂量的肠溶包衣胶囊（3 × 10 - 15）中的疫苗。E）。ASC应答在第3组中最高。S，然后是3 × S。E，2 ×S和1 ×S，按这个顺序。这些差异与在这些方案的田间试验中观察到的疾病保护的差异平行。因此，该测定法可能有助于对新疫苗或疫苗方案的保护能力进行推定性评估。可用于口服疫苗免疫原性的测定。
The immune response to different dosage schedules of oral live Salmonella typhi Ty21a vaccines was studied by enumeration of specific antibody-secreting cells (ASC) in the peripheral blood believed to have been stimulated by the vaccine antigen on mucosal surfaces and to be on their way back to those sites for local antibody secretion. Four groups of subjects were vaccinated with either three (3 .times. S), two (2 .times. S) or one (1 .times. S) dose of a suspension-formulated vaccine, or with three doses of vaccine in enteric-coated capsules (3 .times. E). The ASC-responses were highest in group 3 .times. S, followed by 3 .times. E, 2 .times. S and 1 .times. S, in this order. These differences parallel differences in protection from disease as observed in field trails with these regimens. This assay might therefore be useful for presumptive assessment of the protective ability of new vaccines or vaccine regimens. It certinly can be used to measure the immunogenicity of an oral vaccine.