Continuous-flow isotope ratio mass spectrometry using the chemical reaction interface with either gas or liquid chromatographic introduction.
Continuous-flow isotope ratio mass spectrometry using the chemical reaction interface with either gas or liquid chromatographic introduction.
复制标题
使用化学反应接口并引入气相色谱或液相色谱进行连续流同位素比质谱分析。
DOI：
10.1021/ac951255b
复制
发表时间：
1996
影响因子：
7.4
通讯作者：
Abramson,FP
中科院分区：
文献类型：
--
作者：
Teffera,Y;Kusmierz,JJ;Abramson,FP
介绍了一种引入同位素比质谱仪（IRMS）样品的新方法。该技术利用化学反应界面（CRI）将来自气相色谱仪（GC）或高效液相色谱仪（HPLC）的样品利用微波诱导的氦等离子体转化为二氧化碳。介绍了GC/CRI/IRMS和HPLC/CRI/IRMS的优化参数。在两种操作模式下，都可以得到13co2 / 12co2的比值，标准差小于1‰。在低浓度和高浓度（0.5 ~ 10 μg）条件下，HPLC/CRI/IRMS测定结果无显著差异。利用来自菠菜和藻类的叶绿素来区分不同生物来源的样品的能力得到了证明，两者之间存在较大差异，但保持了良好的精度（SD < 0.60‰）。
A novel method of sample introduction into an isotope ratio mass spectrometer (IRMS) is described. The technique uses the chemical reaction interface (CRI) to convert samples coming from a gas chromatograph (GC) or high-performance liquid chromatograph (HPLC) into CO2using a microwave-induced helium plasma. Optimization parameters for both GC/CRI/IRMS and HPLC/CRI/IRMS are described. In both modes of operation, it was possible to obtain13CO2/12CO2ratios with standard deviations less than 1‰. Investigation of HPLC/CRI/IRMS performance at low and high concentrations (0.5−10 μg) resulted in no significant deviations of the isotope ratios. The ability to differentiate samples of different biological origins was illustrated using chlorophyllafrom spinach and algae, where a large difference was observed but good precision was maintained (SD < 0.60‰).