Coxiella burnetii and Related Tick Endosymbionts Evolved from Pathogenic Ancestors.
Coxiella burnetii and Related Tick Endosymbionts Evolved from Pathogenic Ancestors.
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DOI：
10.1093/gbe/evab108
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发表时间：
2021-07-06
影响因子：
3.3
通讯作者：
Raghavan R
中科院分区：
文献类型：
--
作者：
Brenner AE;Muñoz-Leal S;Sachan M;Labruna MB;Raghavan R
柯克斯体科中的共生菌和致病菌均引起人类和动物的发病和死亡。例如，类柯克斯体内共生体（克莱斯）提高了蜱的繁殖成功率-一种主要的疾病载体，而贝氏柯克斯体引起人类Q热，以及未表征的柯克斯体感染动物和人类。为了更好地了解这组细胞内细菌的发病机制和共生关系的演变，我们对软蜱Ornithodoros amblus（CLEOA）中存在的CLE的基因组进行了测序，并将其与军团菌目中其他细菌的基因组进行了比较。我们的分析证实CLEOA与C. Burnettii，人类病原体，比硬蜱中的克莱斯，并表明大多数克莱斯分支含有内共生体和病原体，表明几个CLE谱系独立于致病性柯克斯体进化而来。我们还确定了CLEOA和C.尽管水平基因转移（HGT）对贝氏梭菌的进化有重要贡献，但贝氏梭菌具有感染巨噬细胞的能力。burnetii，大多数收购事件主要发生在祖先早于CLEOA-C。伯内特分歧这些发现阐明了C.贝氏体，这以前被认为是出现时，无毒蜱内共生体最近获得毒力因子通过HGT。最后，我们确定了几种代谢途径，包括血红素生物合成，这可能是至关重要的人类病原体的细胞内生长，但不是蜱共生体，并表明使用血红素类似物是一个有前途的方法来控制C。贝氏体感染
Both symbiotic and pathogenic bacteria in the family Coxiellaceae cause morbidity and mortality in humans and animals. For instance, Coxiella-like endosymbionts (CLEs) improve the reproductive success of ticks—a major disease vector, while Coxiella burnetii causes human Q fever, and uncharacterized coxiellae infect both animals and humans. To better understand the evolution of pathogenesis and symbiosis in this group of intracellular bacteria, we sequenced the genome of a CLE present in the soft tick Ornithodoros amblus (CLEOA) and compared it to the genomes of other bacteria in the order Legionellales. Our analyses confirmed that CLEOA is more closely related to C. burnetii, the human pathogen, than to CLEs in hard ticks, and showed that most clades of CLEs contain both endosymbionts and pathogens, indicating that several CLE lineages have evolved independently from pathogenic Coxiella. We also determined that the last common ancestorof CLEOA and C. burnetii was equipped to infect macrophages and that even though horizontal gene transfer (HGT) contributed significantly to the evolution of C. burnetii, most acquisition events occurred primarily in ancestors predating the CLEOA–C. burnetii divergence. These discoveries clarify the evolution of C. burnetii, which previously was assumed to have emerged when an avirulent tick endosymbiont recently gained virulence factors via HGT. Finally, we identified several metabolic pathways, including heme biosynthesis, that are likely critical to the intracellular growth of the human pathogen but not the tick symbiont, and show that the use of heme analog is a promising approach to controlling C. burnetii infections.
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影响因子：
6.4
作者：
Beare PA;Gilk SD;Larson CL;Hill J;Stead CM;Omsland A;Cockrell DC;Howe D;Voth DE;Heinzen RA
通讯作者：
Heinzen RA
影响因子：
3.2
作者：
Almeida, Aliny P.;Marcili, Arlei;Labruna, Marcelo B.
通讯作者：
Labruna, Marcelo B.
影响因子：
3.2
作者：
Beare, Paul A.;Sandoz, Kelsi M.;Heinzen, Robert A.
通讯作者：
Heinzen, Robert A.
影响因子：
5.8
作者：
Cohen, Ofir;Ashkenazy, Haim;Pupko, Tal
通讯作者：
Pupko, Tal
影响因子：
3.4
作者：
Bitew, Mebratu A.;Hofmann, Janine;Sansom, Fiona M.
通讯作者：
Sansom, Fiona M.