Reverse-phase high-performance liquid chromatography of proteins: the separation of hemoglobin chain variants.
Reverse-phase high-performance liquid chromatography of proteins: the separation of hemoglobin chain variants.
复制标题
蛋白质的反相高效液相色谱：血红蛋白链变体的分离。
DOI：
10.1016/0003-2697(80)90474-1
复制
发表时间：
1980
影响因子：
2.9
通讯作者：
Böhlen,P
中科院分区：
文献类型：
--
作者：
Petrides,PE;Jones,RT;Böhlen,P
研究了反相高效液相色谱法分离密切相关蛋白质的潜力。用十八硅烷基硅胶固定相和丙醇/甲酸吡啶溶剂系统，将人血红蛋白的正常α-和β-链与其各自的突变链分离，这些突变链仅相差一个氨基酸残基。结果表明，反相高效液相色谱是一个强大的工具，分离中等大小的蛋白质与最小的结构差异。
The potential of reverse-phase high-performance liquid chromatography for the separation of closely related proteins has been investigated. Using an octadecasilyl silica stationary phase and a propanol/pyridine formate solvent system normal α- and β-chains of human hemoglobin have been separated from several of their respective mutant chains which differ by single amino acid residues only. The results suggest that reverse-phase high-performance liquid chromatography is a powerful tool for the separation of medium-sized proteins with minimal structural difference.