Blood-vessel closure using photosensitizers engineered for two-photon excitation
Blood-vessel closure using photosensitizers engineered for two-photon excitation
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DOI：
10.1038/nphoton.2008.100
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发表时间：
2008-07-01
期刊：
影响因子：
35
通讯作者：
Anderson, Harry L.
中科院分区：
文献类型：
--
作者：
Collins, Hazel A.;Khurana, Mamta;Anderson, Harry L.
由双光子激发提供的光吸收的空间控制已经导致了显微镜（1）和微加工（2）的巨大进步。在光动力学疗法中双光子激发的医学应用（3，4）已经被广泛提出（5-18），但迄今为止由于光敏剂药物（其是被活组织吸收的化合物，其在吸收光时变得有毒）的低双光子横截面而变得不切实际。有效的双光子激活药物的发明将允许治疗体积的精确三维操纵，提供当前治疗技术无法达到的靶向水平。在这里，我们提出了一个新的家庭的光动力学治疗药物设计的有效的双光子激发，并使用其中之一，以证明通过双光子激发光动力学治疗在体内选择性关闭血管。这些共轭卟啉二聚体具有比标准临床光敏剂大两个数量级以上的双光子截面（17）。这是第一次证明在体内光动力疗法使用的光敏剂工程有效的双光子激发。
The spatial control of optical absorption provided by two-photon excitation has led to tremendous advances in microscopy(1) and microfabrication(2). Medical applications of two-photon excitation in photodynamic therapy(3,4) have been widely suggested(5-18), but thus far have been rendered impractical by the low two-photon cross-sections of photosensitizer drugs (which are compounds taken up by living tissues that become toxic on absorption of light). The invention of efficient two-photon activated drugs will allow precise three-dimensional manipulation of treatment volumes, providing a level of targeting unattainable with current therapeutic techniques. Here we present a new family of photodynamic therapy drugs designed for efficient two-photon excitation and use one of them to demonstrate selective closure of blood vessels through two-photon excitation photodynamic therapy in vivo. These conjugated porphyrin dimers have two-photon cross-sections that are more than two orders of magnitude greater than those of standard clinical photosensitizers(17). This is the first demonstration of in vivo photodynamic therapy using a photosensitizer engineered for efficient two-photon excitation.