Comparison of the amount of thiotrophic symbionts in the deep-sea mussel Bathymodiolus septemdierum under different sulfide levels using fluorescent in situ hybridization
Comparison of the amount of thiotrophic symbionts in the deep-sea mussel Bathymodiolus septemdierum under different sulfide levels using fluorescent in situ hybridization
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DOI：
10.1007/s12562-011-0419-7
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发表时间：
2012-01-01
影响因子：
1.9
通讯作者：
Inoue, Koji
中科院分区：
文献类型：
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作者：
Fujinoki, Masaru;Koito, Tomoko;Inoue, Koji
栖息在热液喷口的各种无脊椎动物体内含有硫营养内共生体，这些内共生体为宿主提供营养，并可能参与有害硫化物的解毒过程。在这项研究中，我们首先测定了深海贻贝 Bathymodiolus septemdierum（小笠原群岛热液喷口的优势物种）硫营养共生体的部分16S rRNA基因序列。然后，我们利用在所有深海偏顶蛤属贻贝的硫营养共生体中完全保守的区域设计了一种新的探针Bsob692，用于荧光原位杂交（FISH），并建立了FISH操作流程，以便使用市售的图像分析程序比较共生体的分布和数量。我们比较了在不同地点采集的贻贝鳃中的荧光强度，发现在硫化物浓度较高的地点采集的样本具有更高的强度。我们还比较了在有硫化物和无硫化物环境中饲养的贻贝，发现前者具有更高的荧光强度。
Various invertebrates inhabiting hydrothermal vents harbor thiotrophic endosymbionts that provide the host with nutrients and are possibly involved in the detoxification of harmful sulfides. In this study, we first determined the partial 16S rRNA gene sequence of the thiotrophic symbiont of the deep-sea mussel Bathymodiolus septemdierum, a dominant species at hydrothermal vents in the Izu-Ogasawara (Bonin) area. We then designed a new probe, Bsob692, for fluorescent in situ hybridization (FISH) using regions completely conserved among thiotrophic symbionts of all bathymodiolin mussels and established the protocol for FISH to compare the distribution and amount of the symbiont using an image analysis program that is commercially available. We compared fluorescent intensity in the gill of the mussels collected at different sites and found a higher intensity in specimens collected from a site with higher sulfide concentration. We also compared mussels reared in the presence and absence of sulfide and found that the former had a higher fluorescent intensity.