A colorimetric aptamer biosensor based on cationic polymer and gold nanoparticles for the ultrasensitive detection of thrombin
A colorimetric aptamer biosensor based on cationic polymer and gold nanoparticles for the ultrasensitive detection of thrombin
复制标题
基于阳离子聚合物和金纳米颗粒的比色适体生物传感器，用于超灵敏检测凝血酶
DOI：
10.1016/j.bios.2014.01.012
复制
发表时间：
2014-06-15
影响因子：
12.6
通讯作者：
Lin, Yuqing
中科院分区：
文献类型：
--
作者：
Chen, Zhengbo;Tan, Yuan;Lin, Yuqing
以未修饰的金纳米粒子（ununps）为探针，21聚体凝血酶结合适体（TBA）为传感元件，建立了一种基于阳离子聚合物和金纳米粒子的超灵敏凝血酶比色测定方法。加入凝血酶后，TBA与凝血酶特异性相互作用，形成g -四重体结构。结果，构象的改变促进了阳离子聚合物聚二烯基二甲基氯化铵（PDDA）诱导的AuNP聚集。因此，从酒红色到蓝紫色的可见变化很容易被肉眼看到。在其他干扰蛋白存在的情况下，比色传感器可以高选择性地检测低至1pm的凝血酶。此外，该方法已成功地用于测定人血清样品中的凝血酶，这表明它在诊断目的方面具有很大的潜力。(C) 2014 Elsevier B.V.版权所有
A colorimetric assay for the ultrasensitive determination of thrombin based on cationic polymer and gold nanoparticles was presented, in which unmodified gold nanoparticles (AuNPs) was used as probes and 21-mer thrombin-binding aptamer (TBA) as sensing elements. Upon the addition of thrombin, TBA interacted specifically with thrombin to form a G-quadruplex structure. As a result, the conformation change facilitated the cationic polymer, poly(diallyldimethylammonium chloride) (PDDA)-induced AuNP aggregation. Thus, the visible change in color from wine-red to blue-purple was readily seen by the naked eye. The colorimetric sensor could detect thrombin down to 1 pM with high selectivity in the presence of other interferring proteins. Furthermore, the assay was successfully employed to determine thrombin in human serum sample, which suggested its great potential for diagnostic purposes. (C) 2014 Elsevier B.V. All rights reserved.