The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures
The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures
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DOI：
10.1016/j.biomaterials.2010.01.056
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Papaccio, Gianpaolo
中科院分区：
文献类型：
--
作者：
Mangano, Carlo;De Rosa, Alfredo;Papaccio, Gianpaolo
骨组织工程（BTE）和牙科种植学（DI）需要在骨中整合具有良好表征表面的植入结构。在这项工作中，我们用人成骨细胞或人牙髓干细胞（DPSC）挑战酸蚀刻钛（AET）和激光烧结钛（LST）表面，以了解其骨整合和衍生植入物的临床使用能力。DPSC和人成骨细胞用两种钛表面进行挑战，无论是在平面培养中还是在培养室内的辊装置中，持续数小时至一个月。在培养过程中，对钛表面的细胞进行了组织学、蛋白质分泌和基因表达检查。结果表明，使用人DPSC获得了完全的骨整合：这些细胞能够快速分化成成骨细胞和内皮细胞，然后能够沿植入物表面沿着产生骨组织。当用LST表面挑战干细胞时，以更好和更快的方式获得DPSC的成骨细胞分化和骨形态发生蛋白的产生。这种在相对较短的时间内成功的BTE提供了有趣的数据，表明LST是DI临床使用的一种有前途的替代方法。(C)2010爱思唯尔有限公司版权所有。
Bone Tissue Engineering (BTE) and Dental Implantology (DI) require the integration of implanted structures, with well characterized surfaces, in bone. In this work we have challenged acid-etched titanium (AET) and Laser Sintered Titanium (LST) surfaces with either human osteoblasts or stem cells from human dental pulps (DPSCs), to understand their osteointegration and clinical use capability of derived implants. DPSCs and human osteoblasts were challenged with the two titanium surfaces, either in plane cultures or in a roller apparatus within a culture chamber, for hours up to a month. During the cultures cells on the titanium surfaces were examined for histology, protein secretion and gene expression. Results show that a complete osteointegration using human DPSCs has been obtained: these cells were capable to quickly differentiate into osteoblasts and endotheliocytes and, then, able to produce bone tissue along the implant surfaces. Osteoblast differentiation of DPSCs and bone morphogenetic protein production was obtained in a better and quicker way, when challenging stem cells with the LST surfaces. This successful BTE in a comparatively short time gives interesting data suggesting that LST is a promising alternative for clinical use in DI. (C) 2010 Elsevier Ltd. All rights reserved.