Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton
Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton
复制标题
DOI：
10.1007/bf02885512
复制
发表时间：
1998-01-01
影响因子：
--
通讯作者：
Kohel, RJ
中科院分区：
文献类型：
--
作者：
Guo, WZ;Zhang, TZ;Kohel, RJ
采用散装分离分析方法构建两个混合DNA池，筛选与陆地棉育性恢复基因（Rf(i)）连锁的RAPd标记。从（0-613-2R ×类人猿3号）BC3分离群体中分离得到可育和不育DNA，共筛选了425条任意10-mer寡核苷酸引物。三个引物在成对的群体和它们的亲本之间产生了可重复的多态性。用这三种引物对92株（钟12A-1x0-613-2R）F-2进行了DNA提取和扩增。通过雄性育性评分和RAPD扩增，发现一个命名为OPV-15（300）的RAPD标记片段与育性恢复基因Rf(1)连锁，重组值为13.0 +/- 2.57%。
Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertility-restoring gene(Rf(i)) of upland cotton. A total of 425 arbitrary 10-mer oligonucleotide primers were screened on two DNA pools, bulked made fertile and sterile DNAs isolated from BC3 segregating population of (0-613-2R x Simian No. 3). Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of (Zhong 12A-1x0-613-2R)F-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV-15(300) was linked with the fertility-restoring gene (Rf(1)) with a recombination value of 13.0 +/- 2.57%.