Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro.
Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro.
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DOI：
10.1084/jem.161.3.526
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发表时间：
1985-03-01
期刊：
影响因子：
--
通讯作者：
Steinman RM
中科院分区：
文献类型：
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作者：
Schuler G;Steinman RM
鼠表皮兰格汉细胞（LC）已在组织培养中进行了研究，并与脾脏树突状细胞（DC）进行了比较。 LC占起始细胞悬浮液的3％，并通过抗IA和抗MAC-1单克隆抗体的细胞学和反应性与角质形成细胞区分开。 LC不贴心，浮力较低，没有增殖，并且可以富集至10-50％的纯度。 LC在培养物中继续展示IA和MAC-1抗原4天。然而，LC迅速损失的Birbeck颗粒，FC受体，F4/80抗原以及非特异性酯酶和膜ATPase的细胞化学反应性。结果，培养的LC的超微结构和表型与淋巴DC非常相似。每天监测T细胞增殖反应（氧化有丝分裂和混合白细胞反应）的刺激能力。最初，即使LC表示大量的IA抗原，刺激能力也很弱。在培养中2-3 d后，LC的效力比脾脏DC高3-10倍。 30 LC可以在3 x 10（5）响应T细胞的培养物中诱导显着反应。在培养开始时，去除IA+ LC的消除刺激活性的发展，但暴露于1,500 RAD电离辐射却没有。混合实验表明，污染的ia-表皮细胞不会改变IA+刺激剂的功能。因此，LC在免疫学上似乎不成熟，但在培养过程中获得了脾脏DC的许多特征。我们建议，功能性淋巴DC通常是从位于非淋巴组织中的较不成熟的前体来得出的。
Murine epidermal Langerhans cells (LC) have been studied in tissue culture and compared to spleen dendritic cells (DC). LC comprised 3% of the starting cell suspensions and were distinguished from keratinocytes by cytology and reactivity with anti-Ia and anti-Mac-1 monoclonal antibodies. The LC were nonadherent, had a low buoyant density, did not proliferate, and could be enriched to 10-50% purity. LC continued to exhibit Ia and Mac-1 antigens for 4 d in culture. However, LC rapidly lost Birbeck granules, Fc receptors, F4/80 antigen, and cytochemical reactivity for nonspecific esterase and membrane ATPase. As a result, the ultrastructure and phenotype of cultured LC became remarkably similar to lymphoid DC. Stimulatory capacity for T cell proliferative responses (oxidative mitogenesis and the mixed leukocyte reaction) was monitored daily. Initially, stimulatory capacity was very weak, even though LC expressed substantial levels of Ia antigens. After 2-3 d in culture, LC had become 3-10 times more potent than spleen DC. 30 LC could induce significant responses in cultures of 3 X 10(5) responding T cells. Removal of Ia+ LC at the start of culture ablated the development of stimulatory activity, but exposure to 1,500 rad of ionizing irradiation did not. Mixing experiments showed that contaminating Ia- epidermal cells did not alter the function of Ia+ stimulators. Therefore, LC seem to be immunologically immature, but acquire many of the features of spleen DC during culture. We suggest that functioning lymphoid DC may, in general, be derived from less mature precursors located in nonlymphoid tissues.