Isotretinoin produces significant inhibition of monocyte and neutrophil chemotaxis in vivo in patients with cystic acne.
Isotretinoin produces significant inhibition of monocyte and neutrophil chemotaxis in vivo in patients with cystic acne.
复制标题
异维A酸对囊性痤疮患者体内的单核细胞和中性粒细胞趋化性产生显着的抑制作用。
DOI：
10.1111/1523-1747.ep12580370
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Tonnesen,MG
中科院分区：
文献类型：
--
作者：
Norris,DA;Osborn,R;Robinson,W;Tonnesen,MG
在7例囊性痤疮患者中纵向研究了口服异维甲酸（13-顺式维甲酸）对体内趋化反应的影响。如在微室趋化性测定中所测量的，在异维甲酸处理期间，单核细胞和中性粒细胞的趋化性均被抑制98%（p< 0.001）。体内趋化反应在停止治疗后2个月内恢复正常。异维A酸患者的皮肤腔部位活检显示，自体酵母多糖激活血清没有显着的真皮或表皮白细胞积累，而对照组的腔室显示，即使在表皮广泛的嗜中性粒细胞浸润。相反，异维甲酸患者的中性粒细胞和单核细胞的体外趋化反应并没有减弱。异维甲酸患者的血清和血浆中不含趋化性抑制剂，这些患者的活化血清对正常单核细胞是极好的吸引剂。我们推测，异维A酸产生显着的抗炎作用，通过抑制单核细胞和中性粒细胞的趋化性穿过完整的生物屏障在体内。
The effect of oral isotretinoin (13-cis-retinoic acid) on in vivo chemotactic responses was studied longitudinally in 7 patients with cystic acne. As measured in a microchamber chemotaxis assay, both monocyte and neutrophil chemo- taxis were inhibited 98% (p< 0.001) during isotretinoin treatment. In vivo chemotactic responses returned to normal within 2 months of cessation of treatment. Biopsies of skin chamber sites from patients on isotretinoin showed no significant dermal or epidermal leukocytic accumulation in response to autologous zymosan-activated serum, whereas chambers from controls showed extensive neutrophilic infiltrates even in the epidermis. In contrast, in vitro chemotactic responses of neutrophils and monocytes from patients on isotretinoin were not diminished. Sera and plasma from patients on isotretinoin contained no inhibitors of chemotaxis, and activated sera from these patients were excellent attractants for normal monocytes. We postulate that isotretinoin produces significant anti-inflammatory effects by inhibition of monocyte and neutrophil chemotaxis across intact biologic barriers in vivo.