Characterization of hydroperoxides and carbonyl compounds obtained by lipoxygenase extracts of selected microorganisms
Characterization of hydroperoxides and carbonyl compounds obtained by lipoxygenase extracts of selected microorganisms
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DOI：
10.1271/bbb.61.1262
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发表时间：
1997-08-01
影响因子：
1.6
通讯作者：
Kermasha, S
中科院分区：
文献类型：
--
作者：
Bisakowski, B;Perraud, X;Kermasha, S
从增殖镰刀菌、尖孢镰刀菌、蛋白核小球藻和酿酒酵母中获得部分纯化的脂氧合酶 (LOX) 提取物； F. proliferatum 的酶提取物表现出最高的 LOX 活性，而 F. oxysporum 和 S. cerevisiae 在 pH 8.0 时仅表现出酶活性的 27.8% 和 16.5%，在 pH 10.0 时分别表现出酶活性的 61.2% 和 9.7%。以亚油酸为底物，四种提取物将其生物转化为 9- 和 13-氢过氧化物 (HPODE)；此外，尖孢镰刀菌提取物中的LOX活性从亚油酸中产生10-和12-HPODE，而蛋白核棒孢提取物中的LOX活性仅产生10-HPODE。当用9-和13-HPODE作为底物进行测定时，所选微生物提取物具有次级酶活性，其中一种产生己醛。 F. proliferatum 和 C. Pyrenoidosa 提取物中己醛的最高产生活性分别为 1.51 和 1.39 nmol 己醇/mg 蛋白质/分钟，而 F. oxysporum 和 S. cerevisiae 的 HPODE 裂解酶活性约为 15%。 C. Pyrenoidosa 提取物中戊酮的比例最高，其产生浓度仅为 F. oxysporum 和 S. cerevisiae 的约 15%。其他三种微生物酶提取物中的 HPODE 裂解酶活性。
Partially purified lipoxygenase (LOX) extracts were obtained from Fusarium proliferatum, Fusarium oxysporum, Chlorella pyrenoidosa, and Saccharomyces cerevisiae; the enzymatic extract of F. proliferatum showed the highest LOX activity while those of F. oxysporum and S. cerevisiae demonstrated only 27.8 and 16.5% of the activity at pH 8.0, and 61.2 and 9.7% of the enzyme activity at pH 10.0, respectively, The lowest LOX activity was that in the C. pyrenoidosa extract, The microbial enzymatic preparations were assayed with Linoleic acid as substrate, which was bioconverted into 9- and 13-hydroperoxides (HPODEs) by all four extracts; in additon, the LOX activity in the F. oxysporum extract produced the 10- and 12-HPODEs from linoleic acid while that of the C. pyrenoidosa extract produced only the 10-HPODE, When assayed with the 9- and 13-HPODEs as substrates, the selected microbial extracts had secondary enzyme activities, one of which produced hexanal. The highest hexanal-producing activity was 1.51 and 1.39 nmol hexanol/mg protein/min in the F. proliferatum and C. pyrenoidosa extracts, respectively, while those of F. oxysporum and S. cerevisiae had approximately 15% of the HPODE-cleaving enzyme activity, The C. pyrenoidosa extract had the highest proportion of pentanone, which was produced at only one-fourth the concentration by the HPODE-cleaving enzymatic activity in the three other microbial enzymatic extracts.