Synthesis and detoxification of nitric oxide in the plant beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 and its effect on biofilm formation
Synthesis and detoxification of nitric oxide in the plant beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 and its effect on biofilm formation
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植物有益根际解淀粉芽孢杆菌SQR9一氧化氮的合成、解毒及其对生物膜形成的影响
DOI：
10.1016/j.bbrc.2018.06.076
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发表时间：
2018-09-05
影响因子：
3.1
通讯作者：
Zhang, Ruifu
中科院分区：
文献类型：
--
作者：
Dong, Xiaoyan;Liu, Yunpeng;Zhang, Ruifu
一氧化氮(NO)是调节多种生物过程的重要气体信号，由于根际具有很高的氮素循环活性，NO是该区域重要的信号分子。因此，了解NO对根际微生物的影响，特别是对植物有益的根际细菌的影响，对于这些细菌在农业上的应用具有重要意义。研究了外源NO对有益根际细菌淀粉解冻芽孢杆菌SQR9的影响。结果表明，低浓度的NO提高了菌株SQR9形成生物膜的能力，而高浓度的NO抑制了该菌的生长。SQR9基因yflM编码一氧化氮合酶(NOS)，用于合成NO，而基因ykvO编码一种sepiapterin还原酶，用于合成NOS的辅酶四氢生物蝶呤。等温滴定量热法和高效液相色谱分析表明YkvO和NADPH之间存在相互作用。SQR9有两个跛行基因，尽管只有一个被观察到与通过氧化不解毒有关。本研究揭示了NO对植物有益根际细菌的影响，并评价了该菌株对外源NO的适应能力，这将有助于提高该菌株在农业生产中的应用。(C)2018 Elsevier Inc.保留所有权利。
Nitric oxide (NO) is an important gas signal that regulates many biological processes, and due to the high nitrogen recycling activity in the rhizosphere, NO is an important signaling molecule in this region. Thus, an understanding of the effect of NO on the rhizomicrobiome, especially on plant beneficial rhizobacteria, is important for the use of these bacteria in agriculture. In this study, the effect of exogenous NO on the beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 was investigated. The results showed that low concentrations of NO increased the ability of the strain SQR9 to form biofilms, while high concentrations of NO inhibited the growth of this bacterium. The SQR9 gene yflM encodes nitric oxide synthase (NOS), which is used to synthesize NO, while the gene ykvO encodes a sepiapterin reductase that is used to synthesize tetrahydrobiopterin, the coenzyme of NOS. Isothermal titration calorimetry and high-performance liquid chromatography analyses demonstrated an interaction between YkvO and NADPH. SQR9 has two limp genes, although only one was observed to be responsible for NO detoxification through oxidization. This study revealed the effect of NO on plant beneficial rhizobacterium and assessed the ability of this strain to adapt to exogenous NO, which will help to improve the application of this strain in agricultural production. (C) 2018 Elsevier Inc. All rights reserved.