Intraglomerular microcirculation: measurements of single glomerular loop flow in rats.
Intraglomerular microcirculation: measurements of single glomerular loop flow in rats.
复制标题
肾小球内微循环：大鼠单肾小球环流量的测量。
DOI：
10.1038/ki.1981.125
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发表时间：
1981
影响因子：
19.6
通讯作者：
F. Dallenbach
中科院分区：
文献类型：
--
作者：
M. Steinhausen;B. Zimmerhackl;H. Thederan;R. Dussel;N. Parekh;H. Esslinger;G. von Hagens;D. Komitowski;F. Dallenbach
通过使用新的荧光显微镜技术，我们能够测量活体大鼠肾小球单个毛细血管环的平均毛细血管速度和压力。该技术涉及用电视监视器拍摄和记录荧光乳胶颗粒通过肾小球环的流动。在 25 只大鼠中，单个肾小球袢流速为 781 +/- (SD) 271 微米。第 1 节。毛细管环的平均直径测量为 8.4 +/- 1.4 微米；它们的长度为 72.3 +/- 37.5 微米。根据沿着毛细管环的流速的降低，我们能够评估毛细管表面的过滤当量。可以在显微镜控制下连续测量单个肾小球环的毛细血管内压力。高毛细血管内压力与高毛细血管内流速相关。根据我们获得的数据，我们无法计算观察到的毛细管环末端的过滤平衡。为了进一步建立相关性，我们注射了我们研究过的活体肾小球，并用扫描电子显微镜对其进行了检查。
With the use of a new fluorescent microscopic technique, we were able to measure the mean intracapillary velocities and pressures of single capillary loops of renal glomeruli of living rats. The technique involved photographing and recording the flow of fluorescent latex particles through the glomerular loops with a television monitor. In 25 rats the single glomerular loop flow velocity was 781 +/- (SD) 271 micrometers . sec-1. The mean diameter of the capillary loops measured 8.4 +/- 1.4 micrometers; their lengths were 72.3 +/- 37.5 micrometers. From the decrease in velocity of flow along the capillary loop, we were able to evaluate the filtration equivalent for the capillary surface. It was possible to measure intracapillary pressures of single glomerular loops continuously under microscopic control. High intracapillary pressures correlated with high intracapillary velocities. From the data we obtained, we were unable to calculate a filtration equilibrium at the ends of the observed capillary loops. For further correlations, we injected the glomeruli we had studied in the living state and examined them with the scanning electron microscope.