A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum
A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum
复制标题
角铂蛋白 SsCP1 以植物 PR1 为靶点并有助于核盘菌的毒力
DOI：
10.1111/nph.14842
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发表时间：
2018
期刊：
影响因子：
9.4
通讯作者：
Cheng Jiasen
中科院分区：
文献类型：
--
作者：
Yang Guogen;Tang Liguang;Gong Yingdi;Xie Jiatao;Fu Yanping;Jiang Daohong;Li Guoqing;Collinge David B;Chen Weidong;Cheng Jiasen
Cerato‐platanin蛋白（CP）是丝状真菌分泌的一种植物毒素蛋白，其功能尚未完全阐明。利用酵母双杂交、GST下拉、免疫共沉淀和双分子荧光互补等技术，我们发现SsCP 1在质外体中与PR 1相互作用，促进了核盘菌的侵染。过表达PR 1增强了对野生型菌株的抗性，但对Sscp 1敲除菌株没有增强。结果表明，Sscp1基因表达的转基因植株中水杨酸（SA）浓度增加，对灰葡萄孢（Botrytis cinerea）、芸苔链格孢（Alternaria brassicicola）和奥伦格孢（Golovinomyces orontii）等植物病原菌的抗性增强，表明Sscp1基因对油菜菌核病菌的毒力具有重要作用，并能被植物识别，从而引发植物的防御反应。我们的研究结果还表明，SA信号通路参与CP介导的植物防御。
Cerato‐platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date.Here we characterized a CP (SsCP1) from the necrotrophic phytopathogenSclerotinia sclerotiorum.Sscp1transcripts accumulated during plant infection, and deletion ofSscp1significantly reduced virulence. SsCP1 could induce significant cell death when expressed inNicotiana benthamiana.Using yeast two‐hybrid, GST pull‐down, co‐immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection byS. sclerotiorum. OverexpressingPR1enhanced resistance to the wild‐type strain, but not to theSscp1knockout strain ofS. sclerotiorum. Sscp1‐expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namelyBotrytis cinerea,Alternaria brassicicolaandGolovinomyces orontii).Our results suggest that SsCP1 is important for virulence ofS. sclerotiorumand that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP‐mediated plant defense .