Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c‐myc gene.
Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c‐myc gene.
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携带正常人 c-myc 基因的逆转录病毒对鹌鹑胚胎成纤维细胞的转化。
DOI：
10.1002/j.1460-2075.1986.tb04393.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
S. Saule
中科院分区：
文献类型：
--
作者：
P. Martin;C. Henry;F. Ferré;M. Duterque;C. Lagrou;J. Ghysdael;B. Debuire;D. Stéhelin;S. Saule
我们已经构建了表达人类c-myc癌基因的禽类逆转录病毒。这些病毒在转染和感染后在形态上转化原代鹌鹑胚胎成纤维细胞。转化的细胞产生携带剪接的c-myc基因的病毒，并含有高水平的p64 - 67 c-myc蛋白。对其中一种感染性病毒vSX‐AHM进行了分子克隆，并确定了剪接的c-myc插入片段的核苷酸序列。与正常基因组祖细胞相比，在该转化克隆的c-myc编码序列内未发现突变。因此，我们得出结论，诱导原代禽胚胎成纤维细胞转化不需要人c-myc基因突变。
We have constructed avian retroviruses expressing the human c‐myc oncogene. These viruses morphologically transformed primary quail embryo fibroblasts upon transfection and infection. Transformed cells produced viruses harboring a spliced c‐myc gene and contained high levels of p64‐67c‐myc protein. One of these infectious viruses, vSX‐AHM, was molecularly cloned and the nucleotide sequence of the spliced c‐myc insert determined. No mutation was found within the c‐myc coding sequence of this transforming clone when compared to the normal genomic progenitor. Thus, we concluded that no mutation within the human c‐myc gene is required to induce primary avian embryo fibroblast transformation.