Mutations in the amino terminus of herpes simplex virus type 1 gL can reduce cell-cell fusion without affecting gH/gL trafficking.
Mutations in the amino terminus of herpes simplex virus type 1 gL can reduce cell-cell fusion without affecting gH/gL trafficking.
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1 型单纯疱疹病毒 gL 氨基末端的突变可以减少细胞间融合，而不影响 gH/gL 运输。
DOI：
10.1128/jvi.02383-13
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发表时间：
2014
影响因子：
5.4
通讯作者：
Geraghty,RobertJ
中科院分区：
文献类型：
--
作者：
Zhou,Wenbo;Chen,Feng;Klyachkin,Yuri;Sham,YukY;Geraghty,RobertJ
gH/gL异二聚体代表膜融合所必需和足够的四种单纯疱疹病毒糖蛋白中的两种。我们产生了缺失和点突变，覆盖gL残基24至43，以研究该区域在gH/gL细胞内运输和膜融合中的作用。多个突变体显示细胞融合减少40 - 60%，对gH/gL运输没有影响。gL的氨基末端在gH/gL对膜融合的贡献中起重要作用。
The gH/gL heterodimer represents two of the four herpes simplex virus glycoproteins necessary and sufficient for membrane fusion. We generated deletions and point mutations covering gL residues 24 to 43 to investigate that region's role in gH/gL intracellular trafficking and in membrane fusion. Multiple mutants displayed a 40 to 60% reduction in cell fusion with no effect on gH/gL trafficking. The amino terminus of gL plays an important role in the gH/gL contribution to membrane fusion.