The pattern of expression of the 5T4 oncofoetal antigen on normal, dysplastic and malignant oral mucosa
The pattern of expression of the 5T4 oncofoetal antigen on normal, dysplastic and malignant oral mucosa
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DOI：
10.1016/s1368-8375(00)00057-9
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发表时间：
2001-01-01
期刊：
影响因子：
4.8
通讯作者：
Partridge, M
中科院分区：
文献类型：
--
作者：
Ali, A;Langdon, J;Partridge, M
人类5T4癌胚抗原在妊娠期间的所有类型的滋养细胞中都有表达，但在大多数成人组织中没有检测到，尽管在一些上皮细胞上发现了低水平的表达。然而，这种抗原在许多癌症中都有强烈的表达，肿瘤相关标记与胃癌和结肠癌患者的转移扩散和不良的临床预后相关。该基因的过度表达影响了细胞的粘附性、形状和运动性，这可能与5T4癌胎儿抗原在肿瘤发展过程中细胞定位的变化有关。为了确定5T4癌胚抗原是否可以作为口腔癌和癌前病变的肿瘤特异性标志物，我们用免疫组织化学方法研究了正常、炎症和发育不良口腔黏膜组织中5T4的表达模式。口腔粘膜，取自口腔不同部位。以不同的强度和模式表达5T4肿瘤胎儿抗原。免疫反应主要分布在基底层和基底层上，表达延伸到角化部位的棘细胞，当出现炎症时。这种抗原也在下面的结缔组织中被检测到。口腔鳞状细胞癌表现出与正常粘膜相对应的多种染色模式和染色强度。然而，41例中有21例没有间质标记，这一发现也适用于发育不良的病变。5T4癌胚抗原表达模式和强度的改变与肿瘤的临床病理特征无关。这些数据表明，5T4癌胚抗原在正常口腔黏膜上表达，因此该靶标不能用于检测肿瘤或癌前细胞，尽管表达变化可能导致这些病变的发生，(C)2001爱思唯尔科学有限公司。保留所有权利。
The human 5T4 oncofoetal antigen is expressed by all types of trophoblast in pregnancy but is not detected on most adult tissues, although low levels are found on some epithelia. However, this antigen is strongly expressed by many cancers and tumour-associated labelling correlates with metastatic spread and poor clinical outcome for patients with gastric and colon cancer. Overexpression of the gene influences cell adhesion, shape and motility, which may be related to changes in the cellular localisation of the 5T4 oncofoetal antigen as malignancy develops. To establish whether the 5T4 oncofoetal antigen can serve as a tumour-specific marker for oral cancer and precancer, we have evaluated the pattern of expression on biopsies of normal, inflamed and dysplastic oral mucosa using immunohistochemistry. Oral mucosa, taken from different sites in the mouth. expressed the 5T4 oncofoetal antigen with varying intensity and pattern. The majority of the immunoreactivity was detected in the basal and suprabasal layers, with expression extending into the spinous cells at fully keratinised sites and when inflammation was present. This antigen was also detected in the underlying connective tissue. Oral squamous cell carcinoma showed a variety of patterns and intensity of staining corresponding to those found for normal mucosa. However, 21 of 41 cases showed no stromal labelling, a finding also observed for dysplastic lesions. The alterations in the pattern and intensity of 5T4 oncofoetal antigen expression were not related to clinicopathological features of the tumours examined. These data show that the 5T4 oncofoetal antigen is expressed on normal oral mucosa, such that this target cannot be used for detection of neoplastic or preneoplastic cells, although altered expression may contribute to the pathogenesis of these lesions, (C) 2001 Elsevier Science Ltd. All rights reserved.