Afferent mechanisms underlying stimulation modality-related modulation of acupuncture-related cardiovascular responses
Afferent mechanisms underlying stimulation modality-related modulation of acupuncture-related cardiovascular responses
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DOI：
10.1152/japplphysiol.01079.2004
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Longhurst, JC
中科院分区：
文献类型：
--
作者：
Zhou, W;Fu, LW;Longhurst, JC
尽管使用针灸来治疗许多心脏病，但人们对其作用机制知之甚少。因此，我们研究了针灸对麻醉斯普拉格-道利大鼠胃扩张的交感兴奋性心血管反应的影响。在正中神经、腓深神经和尺神经上的 P 5 - 6、S 36 - 37 和 H 6 - 7 处进行 30 分钟的低电流、低频（0.3 - 0.5 mA，2 Hz）电针 (EA)，分别显着降低反射升压反应 40%、39% 和 44%。相比之下，假针灸涉及在 P 5 - 6 处刺针而不进行刺激，或在 LI 6 - 7 穴位进行 30 分钟的电针刺激，覆盖桡神经浅神经，并不会减弱反射。同样，在 P 5 - 6 处使用 40 或 100 Hz 刺激频率的 EA 不会抑制反射。与P 5 - 6 处电针相比，P 5 - 6 和S 36 - 37 两组穴位电针均未导致较大的反射抑制。每 10 分钟在 P 5 - 6 处进行两分钟的手动针灸（MA；2 Hz），持续 30 分钟，可抑制反射性心血管升压反应 33%，该值与 P 5 - 6 处的 2 Hz EA 没有显着差异。电刺激 (ES) 和手动刺激之间的单单位传入活动没有差异。然而，2 Hz ES 比 10 Hz 或 20 Hz ES 激活更多的体细胞传入。这些数据表明，虽然穴位刺激的位置和刺激的频率决定了EA的影响程度，但低频EA和MA在P 5 - 6时几乎没有差异。此外，使用两个独立发挥强作用的穴位同时刺激并不会导致相加或促进相互作用。对 EA 和 MA 反应的相似性以及对高频 EA 心血管反应的缺乏似乎在很大程度上是躯体传入反应的函数。
Despite the use of acupuncture to treat a number of heart diseases, little is known about the mechanisms that underlie its actions. Therefore, we examined the influence of acupuncture on sympathoexcitatory cardiovascular responses to gastric distension in anesthetized Sprague-Dawley rats. Thirty minutes of low-current, low-frequency, (0.3 - 0.5 mA, 2 Hz) electroacupuncture (EA), at P 5 - 6, S 36 - 37, and H 6 - 7 overlying the median, deep peroneal, and ulnar nerves significantly decreased reflex pressor responses by 40, 39, and 44%, respectively. In contrast, sham acupuncture involving needle insertion without stimulation at P 5 - 6 or 30 min of EA at LI 6 - 7 acupoints overlying the superficial radial nerve did not attenuate the reflex. Similarly, EA at P 5 - 6 using 40- or 100-Hz stimulation frequencies did not inhibit the reflex. Compared with EA at P 5 - 6, EA at two sets of acupoints, including P 5 - 6 and S 36 - 37, did not lead to larger inhibition of the reflex. Two minutes of manual acupuncture ( MA; 2 Hz) at P 5 - 6 every 10 min for 30 min inhibited the reflex cardiovascular pressor response by 33%, a value not significantly different from 2-Hz EA at P 5 - 6. Single-unit afferent activity was not different between electrical stimulation (ES) and manual stimulation. However, 2-Hz ES activated more somatic afferents than 10- or 20-Hz ES. These data suggest that, although the location of acupoint stimulation and the frequency of stimulation determine the extent of influence of EA, there is little difference between low-frequency EA and MA at P 5 - 6. Furthermore, simultaneous stimulation using two acupoints that independently exert strong effects did not lead to an additive or a facilitative interaction. The similarity of the responses to EA and MA and the lack of cardiovascular response to high-frequency EA appear to be largely a function of somatic afferent responses.