Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration
Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration
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DOI：
10.18063/ijb.2016.02.009
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发表时间：
2016-01-01
影响因子：
8.4
通讯作者：
Bartolo, Paulo Jorge
中科院分区：
文献类型：
--
作者：
Wang, Weiguang;Caetano, Guilherme Ferreira;Bartolo, Paulo Jorge
支架是细胞附着、增殖和分化的物理基质，最终导致组织再生。它们必须根据特定的生物力学要求进行设计，例如机械性能、表面特性、生物降解性、生物相容性和孔隙率。用于特定组织的支架的最佳设计在很大程度上取决于材料和制造工艺。电活性粒子增强的高分子支架可以通过调控细胞增殖和分化在组织工程中发挥关键作用。本文研究了使用挤出增材制造系统来生产用于骨组织应用的PCL/原始石墨烯支架。使用熔融共混工艺制备PCL/原始石墨烯共混物。用不同浓度的原始石墨烯制备具有规则和可再现结构的支架。从形态学、力学和生物学角度对支架进行评价。结果表明，原始石墨烯的添加改善了支架的机械性能，降低了疏水性，并提高了细胞活力和增殖。
Scaffolds are physical substrates for cell attachment, proliferation, and differentiation, ultimately leading to the regeneration of tissues. They must be designed according to specific biomechanical requirements such as mechanical properties, surface characteristics, biodegradability, biocompatibility, and porosity. The optimal design of a scaffold for a specific tissue strongly depends on both materials and manufacturing processes. Polymeric scaffolds reinforced with electro- active particles could play a key role in tissue engineering by modulating cell proliferation and differentiation. This paper investigates the use of an extrusion additive manufacturing system to produce PCL/pristine graphene scaffolds for bone tissue applications. PCL/pristine graphene blends were prepared using a melt blending process. Scaffolds with regular and reproducible architecture were produced with different concentrations of pristine graphene. Scaffolds were evaluated from morphological, mechanical, and biological view. The results suggest that the addition of pristine graphene improves the mechanical performance of the scaffolds, reduces the hydrophobicity, and improves cell viability and proliferation.