CALCULATION OF FLUORESCENCE RESONANCE ENERGY-TRANSFER ON SURFACES
CALCULATION OF FLUORESCENCE RESONANCE ENERGY-TRANSFER ON SURFACES
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DOI：
10.1016/s0006-3495(80)85033-8
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发表时间：
1980-01-01
影响因子：
3.4
通讯作者：
HAMMES, GG
中科院分区：
文献类型：
--
作者：
DEWEY, TG;HAMMES, GG
提出了一种估算表面上施主和受主分布之间荧光共振能量转移的一般方法。连分数近似从荧光寿命或稳态荧光的量子产率的变化的等效幂级数展开获得。这些近似提供了分析能量传递的解析方程和近似的误差界。针对膜生物化学的5个感兴趣的模型推导出特定的逼近式：无限平面、平行无限平面、球体表面、同心球体表面和2个分离球体的表面。最后，用所得到的方程对文献中的实验结果进行了分析。
A general method for estimating fluorescence resonance energy transfer between distributions of donors and acceptors on surfaces is presented. Continued fraction approximants are obtained from equivalent power series expansions of the change in quantum yield in terms of the fluorescent lifetimes or the steady-state fluorescence. These approximants provide analytic equations for the analysis of energy transfer and error bounds for the approximants. Specific approximants are derived for 5 models of interest for membrane biochemistry: an infinite plane, parallel infinite planes, the surface of a sphere, the surfaces of concentric spheres and the surfaces of 2 separated spheres. Recent experimental results in the literature are analyzed with the equations obtained.