Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy
Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy
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DOI：
10.1210/en.143.3.1144
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发表时间：
2002-03-01
期刊：
影响因子：
4.8
通讯作者：
Anderson, LL
中科院分区：
文献类型：
--
作者：
Cho, SJ;Jeftinija, K;Anderson, LL
早期对活胰腺腺泡细胞的研究发现了细胞质膜上的新细胞结构，称为“凹陷”和“凹陷”，膜结合的分泌囊泡停靠并融合以释放囊泡内容物。在目前的研究中，使用原子力显微镜，我们确定了类似的结构，在质膜的生长激素分泌细胞的垂体，并暗示他们参与激素释放。在静止的GH分泌细胞中鉴定出含有直径为100-200 rim的凹陷或融合孔的凹坑。刺激GH分泌后，凹陷扩大，并发现金标记的GH抗体与刺激的GH细胞中的凹陷结构结合。这项研究首次记录了这些结构的存在及其参与神经内分泌细胞的分泌。
Earlier studies in live pancreatic acinar cells identified new cellular structures at the cell plasma membrane called 'pits' and 'depressions', where membrane-bound secretory vesicles dock and fuse to release vesicular contents. In the current study, using atomic force microscopy we identify similar structures at the plasma membrane of GH-secreting cells of the pituitary and implicate their involvement in hormone release. Pits containing 100-200 rim in diameter depressions or fusion pores were identified in resting GH-secreting cells. Following stimulation of secretion the size of depression enlarged and gold-tagged GH antibody were found to bind to the pit structures in the stimulated GH cells. This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell.