Non-additive gene regulation in a citrus allotetraploid somatic hybrid between C. reticulata Blanco and C. limon (L.) Burm
Non-additive gene regulation in a citrus allotetraploid somatic hybrid between C. reticulata Blanco and C. limon (L.) Burm
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DOI：
10.1038/hdy.2009.162
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发表时间：
2010-09-01
期刊：
影响因子：
3.8
通讯作者：
Ancillo, G.
中科院分区：
文献类型：
--
作者：
Bassene, J. B.;Froelicher, Y.;Ancillo, G.
多倍体植物经常产生新的表型，超出了二倍体基因库中存在的变异范围。通过体细胞杂交已经创造了几百个柑橘异源四倍体杂种。这些基因型是研究异源多倍化对基因表达调节的直接影响的有趣模型。在这里，我们报告了柑橘种间体细胞异源四倍体的果肉全基因组基因表达分析。reticulata cv 'Willowleaf mandarin' + C.利蒙品种“尤里卡柠檬”，使用柑橘20 K cDNA微阵列。两个亲本物种之间的转录组差异约为4%，其中212和160个基因在C. reticulata和C.利蒙。通过定量实时RT-PCR证实某些基因的差异表达。与理论上的中间亲本相比，观察到异源四倍体杂交转录组的整体下调，对于在C. reticulata和C.利蒙。与柠檬相比，柑橘中低表达的基因在异源四倍体中也被系统地抑制。当基因在C. reticulata与C.在利蒙中，异源四倍体基因表达的分布更加平衡。基于基因表达的聚类分析清楚地表明，该杂种更接近C。reticulata比C.利蒙。这些结果表明，有一个全球性的柑橘转录组的优势，与我们以前的芳香化合物和蛋白质组学的研究一致。基因表达的种间分化和非加性基因调控涉及多种生物学途径和不同的细胞成分。Heredity（2010）105，299-308; doi：10.1038/hdy.2009.162; 2009年12月2日在线发表
Polyploid plants often produce new phenotypes, exceeding the range of variability existing in the diploid gene pool. Several hundred citrus allotetraploid hybrids have been created by somatic hybridization. These genotypes are interesting models to study the immediate effects of allopolyploidization on the regulation of gene expression. Here, we report genome-wide gene expression analysis in fruit pulp of a Citrus interspecific somatic allotetraploid between C. reticulata cv 'Willowleaf mandarin' + C. limon cv 'Eureka lemon', using a Citrus 20K cDNA microarray. Around 4% transcriptome divergence was observed between the two parental species, and 212 and 160 genes were more highly expressed in C. reticulata and C. limon, respectively. Differential expression of certain genes was confirmed by quantitative real-time RT-PCR. A global downregulation of the allotetraploid hybrid transcriptome was observed, as compared with a theoretical mid parent, for the genes displaying interspecific expression divergence between C. reticulata and C. limon. The genes underexpressed in mandarin, as compared with lemon, were also systematically repressed in the allotetraploid. When genes were overexpressed in C. reticulata compared with C. limon, the distribution of allotetraploid gene expression was far more balanced. Cluster analysis on the basis of gene expression clearly indicated the hybrid was much closer to C. reticulata than to C. limon. These results suggest there is a global dominance of the mandarin transcriptome, in consistence with our previous studies on aromatic compounds and proteomics. Interspecific differentiation of gene expression and non-additive gene regulation involved various biological pathways and different cellular components. Heredity (2010) 105, 299-308; doi:10.1038/hdy.2009.162; published online 2 December 2009