A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology
A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology
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基于iTRAQ技术的狼疮性肾炎发病、加重和缓解的比较研究
DOI：
10.2174/1386207323666200416151836
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发表时间：
2020-01-01
影响因子：
1.8
通讯作者：
Wu, Yuan-Sheng
中科院分区：
文献类型：
--
作者：
Liao, Dong-Jiang;Cheng, Xi-Ping;Wu, Yuan-Sheng
目的：狼疮性肾炎（LN）是系统性红斑狼疮（SLE）的主要并发症之一。LN的发病、加重和缓解过程的具体机制尚未阐明，但在临床上非常需要。利用iTRAQ技术筛选小鼠LN的功能蛋白。方法：采用腹腔注射LPS、地塞米松、生理盐水（NS）对Mrl-lps小鼠进行干预，以c57小鼠为对照组。抗ANA抗体酶联免疫吸附试验（ELISA）用于验证疾病的严重程度。收集肾组织并进行iTRAQ处理，以筛选出与LN的发生和发展密切相关的功能蛋白。结果：共鉴定出136个蛋白质，其中136个蛋白质具有定量信息。结论：采用iTRAQ方法筛选出与LN发生发展密切相关的功能蛋白Hp、Igkv 8 - 27、Itgb 2、Got 2和Pcx，值得进一步研究。
Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction) were used for verification.Results: We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.