A DNA-mediated transformation system for Dictyostelium discoideum.
A DNA-mediated transformation system for Dictyostelium discoideum.
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DNA 介导的盘基网柄菌转化系统。
DOI：
10.1073/pnas.79.23.7356
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发表时间：
1982
影响因子：
11.1
通讯作者：
Firtel,RA
中科院分区：
文献类型：
--
作者：
Hirth,KP;Edwards,CA;Firtel,RA
建立了盘基网柄藻的转化体系。转化载体含有融合到网骨藻肌动蛋白8基因的拟定启动子的Tn 5新霉素抗性基因的蛋白质编码区;载体还含有在酵母中充当自主复制序列（ars）的序列。使用该载体，我们可以以10（-6）至10（-4）个输入细胞的频率将网骨藻营养阿米巴转化为对氨基糖苷类G418具有抗性。转化的细胞系是稳定的，并且含有整合在染色体DNA内的载体序列。
We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10(-6) and 10(-4) of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.