Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis.
Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis.
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DOI：
10.3389/fpls.2017.01010
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhuo R
中科院分区：
文献类型：
--
作者：
Li Z;Han X;Song X;Zhang Y;Jiang J;Han Q;Liu M;Qiao G;Zhuo R
超氧化物歧化酶（SOD）是一种重要的活性氧清除酶.本研究对景天科植物东南景天（Sedumalfredii）的Cu/ZnSOD基因（SaCu/ZnSOD）的功能进行了研究。Cd胁迫诱导了SaCu/ZnSOD的表达。与野生型（WT）相比，SaCu/Zn SOD基因在转基因拟南芥中的过表达增强了抗氧化防御能力，包括SOD和过氧化物酶活性。此外，它还减少了与过氧化氢（H2O2）和超氧自由基（O2·-）过量产生相关的损害。Cd胁迫对根表离子通量的影响表明，过量表达SaCu/Zn SOD的拟南芥根系具有更强的Cd吸收能力。一个基于基因芯片数据的共表达网络显示了镉诱导的氧化胁迫后拟南芥中可能的氧化调节，表明SaCu/Zn SOD可能参与了这个网络，并增强镉胁迫下ROS的清除能力。总之，这些结果表明，过量表达的SaCu/Zn SOD提高了转基因拟南芥的抗氧化胁迫能力，并为了解SaCu/Zn SOD在响应非生物胁迫中的作用提供了有用的信息。
Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities. Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H2O2) and superoxide radicals (O2•-). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress. Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.