Isolation and identification of the cDNA encoding the pheromone biosynthesis activating neuropeptide and additional neuropeptides in the oriental tobacco budworm, Helicoverpa assulta (Lepidoptera:Noctuidae)
Isolation and identification of the cDNA encoding the pheromone biosynthesis activating neuropeptide and additional neuropeptides in the oriental tobacco budworm, Helicoverpa assulta (Lepidoptera:Noctuidae)
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DOI：
10.1016/s0965-1748(98)00065-4
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发表时间：
1998-10-01
影响因子：
3.8
通讯作者：
Tatsuki, S
中科院分区：
文献类型：
--
作者：
Choi, MY;Tanaka, M;Tatsuki, S
本研究涉及克隆和表征 Has-PBAN cDNA，该 cDNA 长 756 个核苷酸，从 Helicoverpa assulta 成虫的大脑和食管下神经节复合体 (Br-Sg) 中分离出来。从该 cDNA 推导的 194 个氨基酸序列具有蛋白水解内切位点，可产生多个肽。从前激素原的加工过程可以预测，该 cDNA 具有 33 个氨基酸的 PBAN 结构域和 4 个附加肽结构域：24 个氨基酸、7 个氨基酸、18 个氨基酸和 8 个氨基酸长序列，其中 FXPR(或 K)L（X = G、T 或 S）在其 C 末端酰胺化。所有五种预测肽（包括 PBAN）的氨基酸序列与Helicoverpa tea 的氨基酸序列相同（Raina, A.K.、Jaffe, H.、Kempe, T.G.、Keim, P.、Blacher, R.W.、Fales, H.M.、Riley, C.T.、Klun, J.A.、Ridgway, R.L.、Hayes, D.K., 1989。调节雌蛾性信息素产生的神经肽激素。科学 244, 796-798 和 Ma, P.W.K., Knipple, D.C., Roelofs, W.L., 1994。编码信息素生物合成激活神经肽和其他神经肽的前体蛋白的 Helicoverpa 基因的结构组织。 《科学》，美国 91, 506-510）。在1-3日龄雌性和雄性成虫的Br-Sg中检测到了与Has-PBAN cDNA大小相对应的单一mRNA种类，并且它们的表达水平也相似。将雌性或雄性 Br-Sg 提取物或合成 PBAN 注射到断头的 1-3 天龄雌性成虫的血腔中，在光期（当它们不应该产生信息素时）诱导信息素的产生。从这些结果来看，H. assulta 成年雌性似乎使用自己的 PBAN 来调节性信息素生物合成。 Has-PBAN cDNA 中以 FXPR(或 K)L 结尾的四种其他肽以及雄性 PBAN 的功能仍有待阐明。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
The present study is concerned with cloning and characterizing Has-PBAN cDNA which is 756 nucleotides long, isolated from the brain and suboesophageal ganglion complex (Br-Sg) of Helicoverpa assulta adults. The 194-amino acid sequence deduced from this cDNA possessed the proteolytic endocleavage sites to generate multiple peptides. From the processing of the prepro-hormone, it can be predicted that the cDNA has a PBAN domain with 33 amino acids and four additional peptide domains: 24 amino acid-, 7 amino acid-, 18 amino acid- and 8 amino acid-long sequences, with FXPR(or K)L (X = G, T or S) amidated at their C-termini. The amino acid sequence of all five predicted peptides, including the PBAN, are identical to that of Helicoverpa tea (Raina, A.K., Jaffe, H., Kempe, T.G., Keim, P., Blacher, R.W., Fales, H.M., Riley, C.T., Klun, J.A., Ridgway, R.L., Hayes, D.K., 1989. Identification of a neuropeptide hormone that regulates sex pheromone production in female moths. Science 244, 796-798 and Ma, P.W.K., Knipple, D.C., Roelofs, W.L., 1994. Structural organization of the Helicoverpa tea gene encoding the precursor protein for pheromone biosynthesis-activating neuropeptide and other neuropeptides. Proc. Natl. Acad. Sci., U.S.A. 91, 506-510). A single mRNA species corresponding to the size of Has-PBAN cDNA was detected from the Br-Sg of 1-3-day old female and male adults, and their expression was also at a similar level. Pheromone production was induced upon injection of female or male Br-Sg extracts or synthetic PBAN into the haemocoel of decapitated 1-3-day old female adults during the photophase when they are not supposed to produce pheromone. From these results, H. assulta adult females seem to use their own PBAN for regulating sex pheromone biosynthesis. Functions of the four other peptides ending with FXPR(or K)L in the Has-PBAN cDNA and of the male PBAN remain to be elucidated. (C) 1998 Elsevier Science Ltd. All rights reserved.