The modulation of biodistribution of stem cells by anchoring lipid-conjugated heparin on the cell surface
The modulation of biodistribution of stem cells by anchoring lipid-conjugated heparin on the cell surface
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DOI：
10.1016/j.jconrel.2015.08.053
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发表时间：
2015-11-10
影响因子：
10.8
通讯作者：
Tae, Giyoong
中科院分区：
文献类型：
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作者：
Kim, Jong Chul;Tae, Giyoong
肝素是一种具有生物活性的糖胺聚糖，可以与各种细胞外基质（ECM）蛋白和生长因子相互作用。合成了脂质结合肝素，并通过物理插入细胞膜上来涂覆脂肪源性干细胞（ADSC）。脂质缀合的肝素与两个脂质部分的ADSCs上的涂层在体外稳定24小时。通过体内成像系统（IVIS）分析肝素包被的ADSC在小鼠中静脉注射后的生物分布，并且显示在肝脏和脾脏中的积累增加，而在肺中的截留减少。因此，脂肪干细胞的脂质结合肝素涂层可以显着调节细胞的生物分布。(c)2015 Elsevier B.V.版权所有。
Heparin is a bioactive glycosaminoglycan that can interact with various extracellular matrix (ECM) proteins and growth factors. Lipid-conjugated heparin was synthesized, and was used to coat adipose-derived stem cells (ADSCs) by physical insertion on the cell membrane. Coating of lipid-conjugated heparin with two lipid moieties on ADSCs was stable for 24 h in vitro. Biodistribution of heparin-coated ADSCs upon intravenous injection in mice was analyzed by In-Vivo Imaging System (IVIS), and showed enhanced accumulation in the liver and spleen while reduced entrapment in the lung. Thus, the coating of ADSCs with lipid-conjugated heparin could significantly modulate the biodistribution of cells. (c) 2015 Elsevier B.V. All rights reserved.