MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy
MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy
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MiR169及其受ABA调控的靶标PagHAP2-6参与杨树形成层休眠
DOI：
10.1016/j.jplph.2016.03.017
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
He, Xin-Qiang
中科院分区：
文献类型：
--
作者：
Ding, Qi;Zeng, Jun;He, Xin-Qiang
休眠是温带地区多年生植物抵御冬季胁迫的一种有效策略。微小核糖核酸（miRNAs）作为多种生物过程的重要调节因子已广为人知。在本研究中，我们检测了杨树形成层区域在休眠期和活跃生长期miR169成员的表达情况，发现它们具有不同的表达模式。我们鉴定并描述了miR169的一个休眠特异性靶基因PagHAP2 - 6。5' RACE实验证实了miR169对PagHAP2 - 6 mRNA的直接切割。酵母功能互补分析表明，PagHAP2 - 6是杨树中血红素激活蛋白2（HAP2）/核因子Y - A（NF - YA）转录因子的同源物。实时定量聚合酶链反应（qRT - PCR）分析表明，PagHAP2 - 6在休眠期高表达，这与pag - miR169a、n和r的表达模式相反。此外，PagHAP2 - 6的转录受外源脱落酸（ABA）诱导，并且在拟南芥中过表达PagHAP2 - 6以及在本氏烟草中进行瞬时共表达实验均表明，PagHAP2 - 6能够提高对外源ABA的抗性。综上所述，结果表明受ABA调控的miR169及其靶基因PagHAP2 - 6参与了杨树形成层的休眠，这为树木休眠 - 活跃生长转换的调控机制提供了新的见解。（C）2016爱思唯尔集团。保留所有权利。
Dormancy is an effective strategy for perennial plants in temperate zones to survive the winter stress. MicroRNAs (miRNAs) have been well known as important regulators for various biological processes. In this study, we checked the expression of miR169 members in the cambium zone during dormancy and active growth in poplar and found that they had distinct expression patterns. We identified and characterized a dormancy-specific target gene of miR169,PagHAP2-6. 5' RACE assays confirmed the direct cleavage of PagHAP2-6 mRNA by miR169. The yeast functional complementation analysis showed that PagHAP2-6 was a homolog of Heme Activator Protein2 (HAP2)/Nuclear factor Y-A (NF-YA) transcription factor in poplar. qRT-PCR analysis indicated that PagHAP2-6 was highly expressed in the dormant stage, which was converse to the expression pattern of pag-miR169a, n, and r. In addition, the transcription of PagHAP2-6 was induced by exogenous abscisic acid (ABA), and both over-expression of PagHAP2-6 in Arabidopsis and transient co-expression assays in Nicotiana benthamiana indicated that PagHAP2-6 could increase the resistance to exogenous ABA. Taken together, the results suggested that miR169 and its target PagHAP2-6 regulated by ABA were involved in poplar cambium dormancy, which provided new insights into the regulatory mechanisms of tree dormancy-active growth transition. (C) 2016 Elsevier GmbH. All rights reserved.