Augmented brain 5-HT crosses the blood-brain barrier through the 5-HT transporter in rat
Augmented brain 5-HT crosses the blood-brain barrier through the 5-HT transporter in rat
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DOI：
10.1111/j.1460-9568.2008.06201.x
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发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Arita, Hideho
中科院分区：
文献类型：
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作者：
Nakatani, Yasushi;Sato-Suzuki, Ikuko;Arita, Hideho
本研究重新评价了现有的观点，即5-羟色胺（5-HT）不能通过血脑屏障（BBB）穿过大脑进入循环血液。为了单独升高脑5-HT，将5-羟基色氨酸（5-HTP; 30-75 mg/kg）静脉内给予麻醉的大鼠，所述大鼠经历了胃肠道和肾脏切除沿着肝失活（在5-HTP给药后有助于增加血液5-HT的器官）。采用微透析法和高效液相色谱法测定额叶皮质脑组织中5-HT的含量。血液5-HT水平是从全血中测定的，而不是从中心静脉收集的贫血小板血浆。我们发现，血液中的5-HT水平显着增加时，脑5-HT水平显着升高后，给药5-HTP在这些大鼠的腹部手术程序。这种升高在用选择性5-羟色胺再摄取抑制剂（氟西汀; 10 mg/kg i. v.）预处理后消除，尽管脑5-HT水平仍保持增加。这些结果表明，增强的脑5-HT可以通过5-HT转运体从脑到循环血液中穿过BBB。
The present study re-evaluated an existing notion that serotonin (5-hydroxytryptamine; 5-HT) could not cross the brain to the circulating blood via the blood-brain barrier (BBB). To elevate brain 5-HT alone, 5-hydroxytryptophan (5-HTP; 30-75 mg/kg) was administrated intravenously to anaesthetized rats that had undergone gastrointestinal and kidney resections along with liver inactivation (organs contributing to increasing blood 5-HT after 5-HTP administration). A microdialysis method and HPLC system were used to determine the brain 5-HT levels in samples collected from the frontal cortex. Blood 5-HT levels were determined from whole blood, not platelet-poor plasma, collected from the central vein. We found that blood 5-HT levels showed a significant augmentation whenever brain 5-HT levels were significantly elevated after the administration of 5-HTP in those rats with the abdominal surgical procedures. This elevation was abolished after pretreatment with a selective serotonin reuptake inhibitor (fluoxetine; 10 mg/kg i.v.), although brain 5-HT levels remained augmented. These results indicate that augmented brain 5-HT can cross the BBB through the 5-HT transporter from the brain to the circulating blood.