Screening and functional characterization of candidate resistance genes to powdery mildew fromDasypyrum villosum4in a wheat line Pm97033
Screening and functional characterization of candidate resistance genes to powdery mildew fromDasypyrum villosum4in a wheat line Pm97033
复制标题
小麦品系 Pm97033 绒毛白粉病候选抗性基因的筛选及功能鉴定
DOI：
10.1007/s00122-020-03655-4
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发表时间：
2020
影响因子：
5.4
通讯作者：
Ye Xingguo
中科院分区：
文献类型：
--
作者：
Li Shijin;Jia Zimiao;Wang Ke;Du Lipu;Li Hongjie;Lin Zhishan;Ye Xingguo
关键信息在小麦-灰小麦#4T6V#4S·6DL易位系Pm97033中鉴定出的三个基因dvlox、pm21# 4和pm21# 4- h3个基因赋予了小麦抗白粉病的能力。摘要白粉病（PM）是由蓝孢菌引起的。小麦黑穗病是小麦最具破坏性的病害之一。迄今为止，只有少数抗小麦小麦锈病的基因被克隆出来。小麦的野生近缘种，其提供的espm21可使小麦对PM产生免疫。在这项研究中，我们从小麦d中获得了许多差异表达基因（DEGs）。绒毛草#4T6V#4S·6DL易位系Pm97033使用rna测序。其中，与病原菌耐药性相关的7个基因在bgt感染前表达上调。病毒诱导的基因沉默和转化实验表明，其中两个基因dvloxand pm21# 4编码一个脂氧合酶和一个编码卷曲卷曲/核苷酸结合位点/富含亮氨酸的重复抗性蛋白，赋予小麦对PM的抗性。表达dvlox的转基因小麦抗PM增强，表达pm21 #4的转基因小麦抗PM增强。通过簇规则间隔短回文重复相关内切酶（CRISPR/Cas9）系统沉默pm21 #4的Pm97033植株对PM敏感。因此，Pm21#4是Pm97033中产生PM免疫抗性的关键基因。Pm97033和d中沉默的pm21 #4-H的组成性表达。villosum#4赋予了一个对PM敏感的小麦品种Fielder具有PM免疫抗性。
Key messageThree genes designatedDvLox,Pm21#4, andPm21#4-Hidentified in a wheat–Dasypyrum villosum#4T6V#4S·6DL translocation line Pm97033 conferred wheat for powdery mildew resistance.AbstractPowdery mildew (PM) caused byBlumeria graminisf. sp.tritici(Bgt) is one of the most devastating diseases in wheat. To date, only a few genes conferring resistance to wheat PM are cloned.Dasypyrum villosumis a wild relative of wheat, which providesPm21conferring wheat immunity to PM. In this study, we obtained many differentially expressed genes (DEGs) from a wheat–D.villosum#4T6V#4S·6DL translocation line Pm97033 using RNA-sequencing. Among them, 7 DEGs associated with pathogen resistance were up-regulated in front ofBgtinfection. Virus-induced gene silencing and transformation assays demonstrated that two of them,DvLoxandPm21#4encoding a lipoxygenase and a encoding coiled-coil/nucleotide-binding site/leucine-rich repeat resistance protein, conferred wheat PM resistance. The transgenic wheat plants expressingDvLoxenhanced PM resistance, and the transgenic wheat plants expressingPm21#4showed PM immunity. ThePm21#4-silenced Pm97033 plants by the cluster regularly interspaced short palindromic repeats–associated endonuclease (CRISPR/Cas9) system were susceptible to PM. Thus,Pm21#4is a key gene contributing PM immune resistance in Pm97033. Constitutively expression ofPm21#4-H, which is silenced in Pm97033 andD. villosum#4, endowed a PM-susceptible wheat variety Fielder with PM immune resistance.