Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation
Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation
复制标题
DOI：
10.1128/jb.179.15.4676-4683.1997
复制
发表时间：
1997-08-01
影响因子：
3.2
通讯作者：
Scarlato, V
中科院分区：
文献类型：
--
作者：
Beier, D;Spohn, G;Scarlato, V
我们鉴定了幽门螺杆菌的一种新型应激反应操纵子（sro），它包含七个基因，这些基因可能参与多种细胞功能，如趋化性、热休克反应、离子转运和翻译后蛋白修饰。其中三个基因的产物显示出与已知蛋白质的氨基酸同源性，例如鞭毛运动开关蛋白CheY（一类热休克蛋白）和核糖体蛋白L11甲基转移酶，以及与已知蛋白质的氨基酸同源性。磷脂酰转移酶。除了包含功能未知的开放阅读框（其产物预计与膜相关）外，sro 基因座还包含三个开放阅读框，这些开放阅读框之前被描述为构成两个独立的基因座，即幽门螺杆菌的 ftsH 基因和 copAP 操纵子。敲除突变体表明 CheY 对细菌运动至关重要，并且 CopA（而非 CopP）可减轻铜毒性。转录分析表明，该基因座受单个启动子，并且通过向培养基中添加铜以及将温度从 37 摄氏度升高到 45 摄氏度来对转录产生积极影响。讨论了该基因座在幽门螺杆菌毒力中的可能作用。
We identified a novel stress-responsive operon (sro) of Helicobacter pylori that contains seven genes which are likely to be involved in cellular functions as diverse as chemotaxis, heat shock response, ion transport, and posttranslational protein modification, The products of three of these genes show amino acid homologies to known proteins, such as the flagellar motor switch protein CheY, a class of heat shock proteins, and the ribosomal protein L11 methyltransferase, and to a phosphatidyltransferase. In addition to containing an open reading frame of unknown function, the product of which is predicted to be membrane associated, the sro locus contains three open reading frames that have previously been described as constituting two separate loci, the ftsH gene and the copAP operon of H. pylori, Knockout mutants showed that CheY is essential for bacterial motility and that CopA, but not CopP, relieves copper toxicity, Transcriptional analyses indicated that this locus is regulated by a single promoter and that a positive effect on transcription is exerted by the addition of copper to the medium and by temperature upshift from 37 to 45 degrees C. The possible role of this locus in H. pylori virulence is discussed.