Dansyl N-acetyl glucosamine as a precursor of fluorescent chitin: a method to detect fungal cell wall inhibitors.
Dansyl N-acetyl glucosamine as a precursor of fluorescent chitin: a method to detect fungal cell wall inhibitors.
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DOI：
10.7164/antibiotics.50.177
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发表时间：
1997-02
期刊：
影响因子：
--
通讯作者：
L. Carrano;P. Tavecchia;F. Sponga;F. Spreafico
中科院分区：
文献类型：
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作者：
L. Carrano;P. Tavecchia;F. Sponga;F. Spreafico
放射性标记的前体。在C.已经研究了白色念珠菌及其在生长和形态发生中的作用。5 '6 ^几丁质合成酶负责单体单元A，β-乙酰葡糖胺的聚合，导致线性均聚物几丁质。几丁质聚合的抑制影响隔膜形成、细胞壁成熟和芽环形成，损害细胞生长。在这里，我们提出了一种筛选真菌细胞壁抑制剂的方法，该方法需要使用荧光7V-乙酰葡糖胺
radiolabeled precursors. Three different chitin synthase genes (CHS1, CHS2, CHS3) have been identified in C. albicans and their role in growth and morphogenesis have been investigated5'6^ Chitin synthase is responsible for the polymerization of the monomericunit A^-acetylglucosamine leading to the linear homopolymer chitin. Inhibition of chitin polymerization affects septum formation, cell wall maturation and bud ring formation damaging the cell growth. Here, we suggest a method for the screening of fungal cell wall inhibitors, that requires the use of fluorescent 7V-acetyl glucosamine