Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.
Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.
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DOI：
10.1534/genetics.114.165613
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发表时间：
2014-09
期刊：
影响因子：
3.3
通讯作者：
von Korff M
中科院分区：
文献类型：
--
作者：
Pankin A;Campoli C;Dong X;Kilian B;Sharma R;Himmelbach A;Saini R;Davis SJ;Stein N;Schneeberger K;von Korff M
光敏色素在植物光信号和光周期调控开花时间中起着重要作用。在这里，我们建议，红色/远红光感光HvPHYTOCHROME C（HvPHYC），携带GAF结构域的保守区域中的突变，是一个候选人的早熟5位点大麦（大麦L.）。我们精细映射的基因使用映射测序的方法应用于全外显子组捕获数据从bulked早开花分离体来自回交的鲍曼（eam 5）基因渗入线。我们证明eam 5破坏了生物钟基因的昼夜节律表达。此外，它与主效光周期反应基因Ppd-H1相互作用，在非诱导短日照条件下促进开花。我们的研究结果表明，HvPHYC参与光信号的昼夜节律钟的传输，从而调制光依赖的过程，如开花的光周期调节。
Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.
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影响因子：
5.3
作者：
Campoli C;Shtaya M;Davis SJ;von Korff M
通讯作者：
von Korff M
影响因子：
56.9
作者：
Huang, W.;Perez-Garcia, P.;Mas, P.
通讯作者：
Mas, P.
影响因子：
7.4
作者：
Hemming, Megan N.;Peacock, W. James;Trevaskis, Ben
通讯作者：
Trevaskis, Ben
DOI：
10.1073/pnas.1221738110
发表时间：
2013-01-22
影响因子：
11.1
作者：
Hu, Wei;Franklin, Keara A.;Lagarias, J. Clark
通讯作者：
Lagarias, J. Clark
影响因子：
9.2
作者：
Davis, SJ
通讯作者：
Davis, SJ