Karyological sexing and sex ratio of Japanese quail embryos.
Karyological sexing and sex ratio of Japanese quail embryos.
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日本鹌鹑胚胎的核学性别鉴定和性别比。
DOI：
10.1262/jrd1977.31.236
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Akira Okamoto
中科院分区：
文献类型：
--
作者：
M. Yoshizawa;Ikumi Noguchi;T. Muramatsu;Akira Okamoto
本研究在细胞核性别鉴定的基础上，测定了鹌鹑胚胎在孵化16小时时的性比。染色体制备从秋水仙胺引发的胚盘通过空气干燥或火干燥的方法，然后常规Giemsa染色。本文较详细地介绍了一种从鸟类端裂卵制备染色体玻片的方法。通过确定性染色体组成ZZ（雄性）或ZW（雌性）进行性别鉴定，每个胚胎至少有5个中期平板。共制备了305个胚胎用于细胞学性别鉴定。在这274个胚胎中，大约90%可以成功进行性别鉴定。经性别鉴定为雄性的胚胎有138个，雌性的胚胎有136个，性别比为50.4%（雄性），非常接近于50.5%的比例。
The sex ratio of quail embryos at 16 hours of incubation was measured on the basis of karyological sexing. Chromosome preparations were made from Colcemid-primed blastodiscs by the air-or fire-drying method followed by conventional Giemsa staining. A method for preparing chromosome slides from the telolecital eggs of birds was described in some detail. Sexing was made by ascertaining the sex chromosome constitution, ZZ (male) or ZW (female), for a minimum of five metaphase plates per embryo. A total of 305 embryos were prepared for cytological sexing. Of these 274 embryos, about 90% could be sexed successfully. The embryos sexed as male were 138 and those sexed as female were 136, a sex ratio of 50.4 (% males) being very close to the fifty-fifty ratio.