Characterization of non-dominant lethal mutations in the yeast plasma membrane H+-ATPase gene
Characterization of non-dominant lethal mutations in the yeast plasma membrane H+-ATPase gene
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DOI：
10.1016/s0005-2736(98)00247-8
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发表时间：
1999-02-04
影响因子：
3.4
通讯作者：
Portillo, F
中科院分区：
文献类型：
--
作者：
Martín-Castillo, B;Portillo, F
酵母ATP酶的定点突变体先前在将突变体等位基因引入酵母菌株中后进行了研究，其中这些等位基因组成型表达白色，野生型染色体ATP酶基因的表达被关闭。由于功能性H+泵是必不可少的，强选择压力导致在含有低活性变体的细胞生长期间回复突变体的积累。因此，突变基因的组成型表达可以选择反映诸如基因转换或回复等事件的表型。因此，我们重新评估了非显性致死等位基因的表型在一组替代的条件表达系统。我们发现，8个II先前描述的定点突变的行为作为隐性致死等位基因。(C)1999 Elsevier Science B.V. Ali版权所有。
Site-directed mutants of yeast ATPase were previously studied after introduction of mutant alleles into a yeast strain where these alleles were constitutively expressed white the expression of the wild-type chromosomal ATPase gene was turned off. As a functional H+ pump is essential, strong selective pressure leads to the accumulation of revertants during growth of cells harboring variants with low activity. Thus, constitutive expression of the mutant gene can select phenotypes which reflect events such as gene conversion or reversion. We have therefore re-evaluated the phenotypes of non-dominant lethal alleles in an alternative set of conditional expression systems. We show that eight of II previously described site-directed mutations behave as recessive lethal alleles. (C) 1999 Elsevier Science B.V. Ali rights reserved.